FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690852
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATG26300.38068935169906143No Hit
GGATTACCTGTGGTGGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC21950.31772362242564256No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21400.3097624382646355No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACAC21060.304840978965104No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20570.2977482876216614No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20460.29615605078946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17680.2559158835756428No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16480.23854602722435486No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTA16150.23376931672775067No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14820.21451772593840648No Hit
GTTTTACTTCCAATGTTGTATCCACCACAGGTAATCCTGGCCATCTTGCCTGGGGCCACTGACACCGAGGGTGG14210.2056880489598351No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT14150.2048195561422707No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA14130.2045300585364159No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13700.1983058600105377No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13550.19613462796662673No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC13550.19613462796662673No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13190.19092367106124034No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13170.19063417345538552No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12810.18542321654999913No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC12760.18469947253536215No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT12750.18455472373243473No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12240.17717253478313733No Hit
GGATAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12230.17702778598020996No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11910.17239582428653313No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAGGATTAC11900.17225107548360577No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11890.17210632668067835No Hit
GTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTA11780.17051408984847696No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11660.16877710421334816No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11450.16573737935187277No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG11450.16573737935187277No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11070.16023692484063157No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11060.16009217603770418No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC10940.15835519040257537No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGCTATTCGGCG10820.15661820476744656No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10690.15473647032939036No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGCGACTGAAGGTGATTCCAGAGGCGGCACA10650.15415747511768077No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10650.15415747511768077No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10450.15126249905913278No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10200.1476437789859478No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT10120.14648578856252858No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC10060.14561729574496418No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9920.1435908125039806No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9920.1435908125039806No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9740.14098533405128738No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG9710.14055108764250518No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA9710.14055108764250518No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9500.1375113627810298No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA9410.1362086235546832No Hit
TAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCC9170.1327346522844256No Hit
CCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAGGATTACCTGTGGTGGATACAACATTGGAAGTAAAACTGTG8930.12926068101416802No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8910.12897118340831323No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCA8890.12868168580245842No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8870.1283921881966036No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC8660.12535246333512823No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG8640.12506296572927342No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT8550.12376022650292683No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8460.12245748727658023No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8460.12245748727658023No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGG8420.12187849206487063No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC8390.12144424565608841No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG8330.12057575283852404No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8260.11956251121803221No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8240.11927301361217743No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8210.11883876720339523No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8210.11883876720339523No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTTCCAATGTTGTATCCACCACAGGTAATCCTGGC8200.11869401840046782No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG8130.11768077677997604No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAG7850.11362781029800884No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7790.11275931748044445No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGG7740.11203557346580743No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7680.11116708064824304No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7650.11073283423946084No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7480.10827210458969504No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC7240.10479813331943745No Hit
GCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC7220.10450863571358265No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7110.10291639888138125No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7070.10233740366967164No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT6960.10074516683747026No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6940.10045566923161545No Hit
GGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGG6920.10016617162576066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA200.00746882251.55429537
ACACGAG200.007699859351.1585824
GTAATTG450.009818948.1004970
GGTATCA4150.046.0922661
GTATCAA12400.043.523471
TATCAAC13700.038.6147232
ACTATTT750.001517506438.48039270
GTGGTAT901.364242E-1037.952961
TCAACGC14350.037.06054
ATCAACG14400.036.934493
CAACGCA14450.036.5681045
AACGCAG14600.036.19246
ACGCAGA15100.034.768217
GTATAGG400.002725688334.157661
ACGGGTA701.0655676E-634.10078416
AGAGTAC16050.033.1326911
CAGAGTA16000.033.0231810
AATGGTA2559.276846E-1131.12384670
TAGCAAG450.00489907630.303035
GACAGCT1254.611138E-927.326131
Produced by FastQC (version 0.11.5)