FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763636
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29760.3897144712926054No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29510.3864406602098382No Hit
GTCCCAACCAGGACACAACATGGACATGAGGGTCCCTGCTCAGCTCCTGG23660.30983348087308615No Hit
AGCCAGGGCAAGCCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAA21150.2769644176021036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20940.2742144162925792No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACCGAGCTCAGCCTCAGGGAGA19500.2553572644558402No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18460.24173821035152876No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.23898820904200432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18080.2367620175057226No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16570.21698819856580884No Hit
GCACAAGCCAGGGCAAGCCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAAAAAGGGGTCCCATCGAGGTT16390.21463105458621648No Hit
ATATTACTGTCAACACTATGATAATCTCCTTCTTAATTTCGCCGGAGGGA16130.21122629106013863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15820.20716676531750727No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15530.20336914446149734No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGGAACTATTTAAATTGGTATCAGCACAAG15340.2008810480385943No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15240.19957152360548744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15180.1987858089456233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14650.1918453294501569No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13720.17966675222226294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13500.17678579846942785No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGGAA13090.17141674829368966No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12940.16945246164402936No Hit
CAACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12590.1648691261281553No Hit
GTTTATTACTGTGCGAGAGTTCGGAAGAGTGGTTATTTTCCAGCCTACTT12570.16460722124153393No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAAAATTCTGTCCTAGATCCACTTCCA12450.16303579192180567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12450.16303579192180567No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12290.16094055282883468No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11800.154523883106611No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11710.15334531111681482No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11530.15098816713722246No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11420.1495476902608049No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11380.14902388048756213No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGGCGAAATTA11370.14889292804425144No Hit
CCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAAAAAGGGGTCCCA11170.1462738791780377No Hit
ATCTCGGGTGGCTCCATCAGTCCTTCCTACTGGAGCTGGATCCGGCAGTC10900.14273816320864915No Hit
ATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAA10760.14090482900229953No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAAAATTCTGTCCTAGATCCACTTCCA10750.14077387655898882No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10650.13946435212588196No Hit
ACATATTACTGTCAACACTATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAACGAA10490.13736911303291097No Hit
GCCCTGGCTTGTGCTGATACCAATTTAAATAGTTCCTAATGTCTTGACTCGCCTGGCAAGTGATGGTGACT10340.13540482638325066No Hit
GGCTTGTGCTGATACCAATTTAAATAGTTCCTAATGTCTTGACTCGCCTGGCAAGTGATGGTGACT10290.13475006416669721No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10270.13448815928007585No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9980.13069053842406592No Hit
ACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9930.13003577620751247No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9890.12951196643426974No Hit
GGACACAACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT9660.1265000602381239No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9550.12505958336170636No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9520.1246667260317743No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9260.12126196250569643No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAGGGG9100.11916672341272543No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8860.11602386477326894No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8830.11563100744333688No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8560.11209529147394832No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8510.11144052925739488No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8450.11065481459753075No Hit
GTTTAGGGGCTTGCCCTGGCTTGTGCTGATACCAATTTAAATAGTTCCTA8440.11052386215422007No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8430.11039290971090938No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8390.10986909993766664No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8320.10895243283449182No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAG8150.10672624129821015No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8150.10672624129821015No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8050.10541671686510327No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8000.10476195464854984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7990.10463100220523916No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7960.1042381448753071No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7950.10410719243199641No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7930.10384528754537502No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7930.10384528754537502No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7900.10345243021544295No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7860.1029286204422002No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7860.1029286204422002No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7850.10279766799888952No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7850.10279766799888952No Hit
AGTCAAGACATTAGGAACTATTTAAATTGGTATCAGCACAAGCCAGGGCA7820.10240481066895746No Hit
CTATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7760.10161909600909333No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAGGGGTCGTGTCACCATATCTGTGGACACGTCCAAGAA7750.10148814356578265No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7720.10109528623585058No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7680.10057147646260783No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7650.10017861913267578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA150.00236241168.9470838
CGTCTCG150.002415456868.5620831
CGTATGC803.274181E-1143.23187341
TCGTATG856.548362E-1140.6403440
GTATCAA17250.040.0278971
TATCAAC19950.034.7705352
GCCGTCT1003.5106495E-1034.7675446
CACAGAT9650.034.5911270
ATCAACG20400.034.003543
TCAACGC20500.033.837674
ACGTGTC5600.033.83141763
GACGTGT5650.033.4541162
CAACGCA20750.033.4299855
AACGCAG20950.033.1108446
GATTCAC1350.032.9162251
ATCTCGT1056.566552E-1032.7886237
TAGGCAT1056.9485395E-1032.6186129
CCGAGCT5600.032.42751330
ACCGAGC5600.032.41474529
TTGGACG5800.032.3369759
Produced by FastQC (version 0.11.5)