FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763636
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30020.39311923481868327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25370.3322263486792137No Hit
GTCCCAACCAGGACACAACATGGACATGAGGGTCCCTGCTCAGCTCCTGG22080.28914299482999756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20420.26740488924042344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20180.2642620306009669No Hit
AGCCAGGGCAAGCCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAA19490.25522631201252954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17730.23217868198984856No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACCGAGCTCAGCCTCAGGGAGA17420.22811915624721726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16420.21502391191614853No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16330.21384533992635235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.20900009952385692No Hit
ATATTACTGTCAACACTATGATAATCTCCTTCTTAATTTCGCCGGAGGGA15680.20533343111115765No Hit
GCACAAGCCAGGGCAAGCCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAAAAAGGGGTCCCATCGAGGTT15150.19839295161569126No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15090.19760723695582713No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGGAACTATTTAAATTGGTATCAGCACAAG14820.19407152098643857No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14490.1897500903571859No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14190.18582151705786526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13900.18202389620185533No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13660.17888103756239884No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13490.17665484602611717No Hit
GTTTATTACTGTGCGAGAGTTCGGAAGAGTGGTTATTTTCCAGCCTACTT12970.1698453189739614No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGGAA12970.1698453189739614No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12330.16146436260207744No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12180.15950007595241714No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAAAATTCTGTCCTAGATCCACTTCCA12100.15845245640593164No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12010.15727388441613543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11840.15504769287985376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11760.15400007333336826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11500.15059530980729038No Hit
CAACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11260.1474524511678339No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11240.1471905462812125No Hit
GGCTTGTGCTGATACCAATTTAAATAGTTCCTAATGTCTTGACTCGCCTGGCAAGTGATGGTGACT11170.1462738791780377No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10930.1431310205385812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGGCGAAATTA10870.14234530587871708No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAAAATTCTGTCCTAGATCCACTTCCA10820.14169054366216363No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10820.14169054366216363No Hit
ATCTCGGGTGGCTCCATCAGTCCTTCCTACTGGAGCTGGATCCGGCAGTC10750.14077387655898882No Hit
ACATATTACTGTCAACACTATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAACGAA10660.13959530456919264No Hit
ATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAA10620.1390714947959499No Hit
CCCCTAAACTCCTGATCTACGATGCATCCTACTTGGAAAAAGGGGTCCCA10510.13763101791953233No Hit
GGACACAACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT10330.13527387393993998No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10200.13357149217690104No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAGGGG10080.13200006285717278No Hit
GCCCTGGCTTGTGCTGATACCAATTTAAATAGTTCCTAATGTCTTGACTCGCCTGGCAAGTGATGGTGACT9830.1287262517744056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9780.1280714895578522No Hit
ACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9460.12388101137191018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9310.12191672472224988No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9140.1196905331859682No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8960.1173333892063758No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8880.1162857696598903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.11602386477326894No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8830.11563100744333688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8740.1144524354535407No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8430.11039290971090938No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAG8420.11026195726759869No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8340.10921433772111319No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8270.1082976706179384No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8210.10751195595807427No Hit
CTATGATAATCTCCTTCTTAATTTCGCCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8120.10633338396827809No Hit
GTTTAGGGGCTTGCCCTGGCTTGTGCTGATACCAATTTAAATAGTTCCTA8060.10554766930841396No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8060.10554766930841396No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8010.10489290709186053No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7960.1042381448753071No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7920.10371433510206432No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7850.10279766799888952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)