FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171301
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38850.33168246249256167No Hit
GTAGTACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28120.24007492523271134No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23400.19977785385652366No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16750.1430033782947338No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15880.13557574013852972No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTCCATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGG13230.11295132506503452No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13230.11295132506503452No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13060.111499947494282No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12540.1070604396308037No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11880.10142567965023509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.042.5265431
CCTAACG405.5020402E-542.4342732
CGTCTAA350.001242969440.08337850
CAATGCG400.002367468335.15521251
GTGGTAT3450.034.4600261
CTAACGC502.052325E-433.9314733
GTATCAA29850.032.3177151
TGGTATC3950.030.9394192
TATCAAC32000.029.5979082
TTCGATC703.8840353E-529.53445839
TCAACGC33150.028.6527214
ATCAACG33300.028.632863
GTGCTAG3100.028.489071
CAACGCA33600.028.4696875
AACGCAG35050.027.485476
CGCAGAG35350.027.444138
AGAGTAC36350.026.59013711
CAGAGTA37050.026.27307510
GCAGAGT37250.026.2264239
TCCGAGA7350.025.87575770
Produced by FastQC (version 0.11.5)