FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230819
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53780.43694483104339465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50620.41127086923422534No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36820.29915040310557445No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCATG33350.27095779314423973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32680.26551426326697913No Hit
CTACAAACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG32160.26128943410850825No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30740.2497524006372992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30380.246827518912204No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30040.2440651306162807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29780.24195271603704524No Hit
GGTCTAGTCAGAGCCTCATGCAAAGTAGTGGATACAACTGTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC28350.23033443585125027No Hit
GTAGTGGATACAACTGTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT27380.22245350453641033No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26230.21311013235902274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25890.21034774406309947No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25600.20799158934010606No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25050.2035230200378772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24380.19807949016061663No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23700.19255471356877005No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATTCTAAT23330.18954858512908884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23270.18906110484157296No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT23030.18711118369150948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22270.18093643338297508No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT22270.18093643338297508No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGTCTGATCCACTGCCA21950.17833653851622375No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20800.16899316633883618No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20750.16858693276590628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20040.16281841603030178No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19940.16200594888444197No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19850.16127472845316818No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19800.16086849488023827No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT19400.15761862629679912No Hit
GGCCAAAAGTGTGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA19230.15623743214883748No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTATTCGGGCC19010.15445000442794596No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18750.15233758984871051No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17870.14518787896514435No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17860.14510663225055837No Hit
GGATACAACTGTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTATT17700.1438066848171827No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17320.14071930966291551No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16950.13771318122323428No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16950.13771318122323428No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16860.13698196079196048No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCACACTTTTGGCCAGGGGA16810.13657572721903058No Hit
GTTCAGTGGCAGTGGATCAGACACAGATTTTACACTGAAAATCAGCAGAG16600.13486954621272504No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA16540.13438206592520915No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16440.13356959877934935No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16430.13348835206476337No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16260.13210715791680175No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCACTGTGAAGTGCAGCTGGTGGAGT16170.13137593748552792No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGGGTTTATTACTGCATGCAAGCTCTACAAACT15960.12966975647922238No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15940.12950726305005042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15920.12934476962087846No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA15810.1284510557604327No Hit
CAGTAATAAACCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGTCTGATCCACTGCCA15720.12771983532915887No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15480.1257699141790954No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15460.12560742074992343No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15390.12503869374782156No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15330.1245512134603057No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15170.12325126602693004No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14910.1211388514476946No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14810.1203263843018348No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA14660.11910768358304512No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14630.11886394343928718No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14560.11829521643718532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14390.11691402228922368No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14370.11675152886005172No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14360.11667028214546575No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14290.11610155514336389No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT14000.11374540042037051No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13920.11309542670368267No Hit
ATATTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCACACTTTT13690.11122675226820516No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGACACAGATTTTACACTGAA13610.11057677855151732No Hit
AGTCAGAGCCTCATGCAAAGTAGTGGATACAACTGTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG13440.10919558440355569No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13310.10813937711393795No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13300.10805813039935197No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13160.10692067639514827No Hit
GTATTACTGTGCAAAAGACCTTAGCGTTTTGGAATTACCCCGGGGCCACTACTACTACGGTATGGACGTCTGGGG13080.10627070267846045No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13060.10610820924928847No Hit
GAGTGGAGGCTGAGGATATTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCACACTTTTGGCCAGGGGA13000.1056207289617726No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12910.10488950853049879No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12720.10334582095336521No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12700.10318332752419324No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12660.10285834066584934No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12580.1022083669491615No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12560.10204587351998955No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12530.1018021333762316No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12470.10131465308871573No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12450.10115215965954376No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12370.10050218594285594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG200.00751526251.4759726
GGTATCA11450.044.0258061
TCGTATG1400.041.99212340
GTATCAA28300.039.5026861
GTGGTAT3150.039.1910931
TAGCGCG451.03911894E-438.094714
CGCGCGT451.0408606E-438.0838787
TGGTATC3650.035.695672
CTCTATA400.002674467234.2922061
GGCTAGC400.002674467234.2922061
CGTATGC1750.033.62539741
ATCTCGT1750.033.49759337
TATCAAC34700.031.8164162
CTTATAC1950.031.6543451
ATCAACG35100.031.5502363
ATGCCGT1900.031.04335444
TCAACGC35700.031.0199814
CAACGCA36100.030.5812955
GAATACG902.111392E-730.4695789
ACGAGAC2050.030.11145422
Produced by FastQC (version 0.11.5)