FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329328
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59710.44917431965624743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56350.4238983907658606No Hit
GAATATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTT46890.35273461478280754No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42610.3205378958391007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38190.28728801319162767No Hit
GTCTACAAACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAA37320.28074335303250963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35930.27028694197368897No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG33770.25403813054415464No Hit
GATTTTACACTGAATATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGGTCTACAAACT33530.2522327070519842No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33460.2517061252001011No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32740.24628985472358966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32120.2416258440354826No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG32010.24079835826823778No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31450.2365857034531733No Hit
GCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGT30900.2324482746169493No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30780.23154556287086409No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30690.23086852906130015No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30490.2293640094844914No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30220.22733290805579962No Hit
GTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTT29760.22387251302913952No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29550.2222927674734904No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29130.21913327636219204No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAGAGCCTCTTT29100.21890759842567073No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28050.21100887064742485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26350.19822045424455065No Hit
GTTTGTAGACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAAT25690.1932555396410818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCC25060.1885163029741343No Hit
CAATAATACACACCCGTGTCGTCAGGTCTCAGGCTGTTCATCTGCAGATA24810.18663565350312336No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCTCAGGAT23670.1780598919153136No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23270.17505085276169613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22710.1708381979466317No Hit
GGTCTGGTCAGAGCCTCTTTCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTC22540.16955935630634428No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCT21890.16466966768171587No Hit
AGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTATCGGGCC21860.16444398974519456No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21740.16354127799910934No Hit
GTCTCCACTCTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCT21600.1624881142953432No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA21520.1618863064646197No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAACCAATTTGATCAGGAGCTGTGGAGCCTGCCCTGGCTTCTGCAAGTA21240.1597799790570875No Hit
GGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20950.15759842567071483No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20820.15662048794578914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20320.15285918900376733No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20300.15270873704608642No Hit
GTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCCCTTGGCCGAACGTC20230.1521821551942034No Hit
GCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCTCAGGATCCAGAG19650.14781904842145807No Hit
GTTTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAA19460.14638975482348976No Hit
GTATTATTGTGTGTCGACGGCGGTGTCCTTTGCTACTTTTGTCATCTACTACTATTACGGGATGGAAGTCTGGGG19370.14571272101392582No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19020.14307981175451054No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18880.14202664805074441No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCAT18280.13751308932031825No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17670.1329243046110516No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17590.13232249678032812No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17400.1308932031823598No Hit
CTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTG17260.12984003947859368No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17190.12931345762671062No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16870.12690622630381665No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16740.125928288578891No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16730.12585306260005055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16700.12562738466352924No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGAGTTTGTAGACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCT16670.12540170672700793No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16610.12495035085396532No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16380.12322015334063528No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16370.12314492736179483No Hit
CCACTCTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG16360.12306970138295438No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16000.12036156614469867No Hit
CTCCAGGGGAGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATATCC16000.12036156614469867No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAATATTAGCAGAG15940.11991021027165606No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT15800.11885704656788994No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15490.11652504122383639No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15480.11644981524499597No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15330.1153214255623894No Hit
CAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTATCGGGC15260.11479484371050637No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15240.11464439175282548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15230.11456916577398506No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15130.11381690598558068No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15120.11374168000674026No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15060.11329032413369762No Hit
GGTCAGAGCCTCTTTCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAG15010.11291419423949543No Hit
GGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTAT14900.11208670847225063No Hit
TCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGC14870.11186103053572934No Hit
CCACAGCTCCTGATCAAATTGGTTTCTTATCGGGCCTCCGGGGTCCCTGA14770.11110877074732496No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14740.11088309281080365No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14470.10885199138211187No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14440.10862631344559055No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCA14380.10817495757254794No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14190.10674566397457963No Hit
ATCCAGTACTATGAAAGAGGCTCTGACCAGACCTGCAGGAGATGGAGGCC14190.10674566397457963No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14170.10659521201689875No Hit
TTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTTCGGCCAAGGGA14090.10599340418617528No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGAT13870.10433843265168566No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTTCGGCCAAGGGA13610.10238255720183431No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13560.10200642730763212No Hit
ATATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAG13480.10140461947690863No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13450.10117894154038733No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13410.10087803762502556No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13340.10035145577314251No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13330.10027622979430209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.044.055071
TCGTATG1600.043.19072740
GTGGTAT3700.040.570161
CGCTAAT1700.038.27776732
TCGCTAA1800.038.0323731
AATCTCG1750.037.30386736
CGTATGC1900.036.41137341
ATCTCGT1800.036.30754537
TGGTATC3900.035.85862
GTATCAA31800.035.295921
ACACTCG2000.034.15950827
CGAGACA2150.033.3362223
ATCTCCG2050.033.28121610
GTATGCC2300.033.1159142
ACTCGCT2100.032.55739629
CTCGCTA2150.031.81223930
ACGAGAC2200.031.02254522
TATCAAC36350.030.5905462
CTAATCT2150.030.31766734
GCTAATC2150.030.29359833
Produced by FastQC (version 0.11.5)