FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329328
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58970.44360759722205506No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45600.3430304635123912No Hit
GAATATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTT42170.31722795277012145No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41640.3132409758915783No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG36270.2728446252542638No Hit
GTCTACAAACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAA35940.2703621679525294No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35650.26818061456615677No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33560.2524583849885055No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33480.251856577157782No Hit
GATTTTACACTGAATATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGGTCTACAAACT32650.24561282091402573No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG32610.245311916998664No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32090.24140016609896126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30290.2278594899076827No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCA30140.22673110022507614No Hit
GCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGT29080.21875714646798985No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28870.2171774009123407No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28050.21100887064742485No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27980.21048228879554182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27780.20897776921873307No Hit
GTTTGTAGACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAAT27680.20822550943032872No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27280.20521647027671122No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27210.2046898884248282No Hit
GTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTT27120.20401285461526425No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26580.19995065175788068No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25540.19212714995847527No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24870.187087009376166No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAGAGCCTCTTT24650.1854320378416764No Hit
CAATAATACACACCCGTGTCGTCAGGTCTCAGGCTGTTCATCTGCAGATA24090.18121938302661195No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24000.180542349217048No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCC23920.1799405413863245No Hit
GGTCTGGTCAGAGCCTCTTTCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTC23230.17474994884633438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22960.1727188474176426No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22780.17136477979851475No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCTCAGGAT21880.16459444170287543No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21590.16241288831650277No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21140.15902771926868314No Hit
GTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCCCTTGGCCGAACGTC20570.15473983847477824No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCT20560.1546646124959378No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA20130.15142989540579901No Hit
CTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTG20070.1509785395327564No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAACCAATTTGATCAGGAGCTGTGGAGCCTGCCCTGGCTTCTGCAAGTA19980.15030150572319245No Hit
GTCTCCACTCTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCT19720.14834563027334113No Hit
GGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19510.14676588471769195No Hit
GTTTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAA19080.14353116762755316No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18290.13758831529915866No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18260.13736263736263737No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18090.13608379572234994No Hit
GTATTATTGTGTGTCGACGGCGGTGTCCTTTGCTACTTTTGTCATCTACTACTATTACGGGATGGAAGTCTGGGG17860.1343535982090199No Hit
AGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTATCGGGCC17690.13307475656873247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17500.13164546297076418No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17420.13104365514004068No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGAGTTTGTAGACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCT17390.13081797720351937No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17340.13044184730931718No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17060.12833551990178496No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16950.12750803413454018No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16940.1274328081556997No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCAT16680.12547693270584837No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16530.12434854302324182No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16520.12427331704440137No Hit
CCACTCTCCCTGTCCGTCTCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG16470.1238971871501992No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16090.1210385999542626No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15770.11863136863136862No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15590.11727730101224076No Hit
GCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGATCTCAGGATCCAGAG15590.11727730101224076No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15570.1171268490545599No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15130.11381690598558068No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAATATTAGCAGAG15110.11366645402789981No Hit
CTCCAGGGGAGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATATCC15100.11359122804905938No Hit
GGGTCTCTGGCTTGCAATTCATGATCTCAGGATCCAGAGCGGATATTGTGATGACTCAGTCTCCACTCTCC14780.11118399672616538No Hit
GGTCAGAGCCTCTTTCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAG14760.11103354476848452No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14710.11065741487428235No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14640.1101308330223993No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14430.10855108746675013No Hit
TTATTACTGCATGCAGGGTCTACAAACTCCTCCGACGTTCGGCCAAGGGA14310.10764837572066488No Hit
GGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTAT14290.107497923762984No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14250.10719701984762227No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTTGCAATTCATGAT14200.10682088995342008No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14160.10651998603805833No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14010.10539159635545177No Hit
CCACAGCTCCTGATCAAATTGGTTTCTTATCGGGCCTCCGGGGTCCCTGA13980.10516591841893046No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13920.10471456254588785No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13910.1046393365670474No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATATTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13760.10351094688444086No Hit
TCATAGTACTGGATACAATTATTTGGATTGGTACTTGCAGAAGCCAGGGC13580.102156879265313No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13570.10208165328647256No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13500.1015550714345895No Hit
ATCCAGTACTATGAAAGAGGCTCTGACCAGACCTGCAGGAGATGGAGGCC13430.10102848958270644No Hit
CAATTATTTGGATTGGTACTTGCAGAAGCCAGGGCAGGCTCCACAGCTCCTGATCAAATTGGTTTCTTATCGGGC13390.1007275856673447No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13340.10035145577314251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)