FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498400
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32850.21923384943940202No Hit
GTATTATAGTAGTCTTCGCTCTTTCGGCGGAGGGACCAAGGTGGCGATCAAACGAACTGTGGCTGCACCAT32430.21643085958355576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29820.1990122797650828No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28110.18760010678056593No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27580.18406300053390282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24260.16190603310197543No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC23650.15783502402562732No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23610.15756807261078484No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22830.1523625200213561No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20230.1350106780565937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20050.13380939668980246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19890.13274159103043245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18560.12386545648691938No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17790.11872664175120128No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17110.1141884676988788No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17050.11378804057661504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16850.11245328350240255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16490.11005072076882008No Hit
GATCTACACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCA16470.10991724506139883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42450.035.9516941
TACGTAT800.002217976835.62389870
ATCAACG50600.029.9278683
TATCAAC51150.029.8128342
TCAACGC50850.029.7737754
GGTATCA26700.029.5912461
CAACGCA51950.029.078435
GTGGTAT10450.028.7562581
AACGCAG52500.028.6453446
ATTGCCG1050.00839070627.14201770
ACGCAGA55950.026.9392787
CGCAGAG56350.026.8078868
AGAGTAC58300.026.08212711
CAGAGTA58950.025.73992210
GCAGAGT59300.025.5311369
GACAGGT3850.025.4329031
TTAACGG1351.0657459E-825.26392635
GCGATCA13250.024.88877344
CGGTACC1401.2727469E-824.84557245
TAACGGC1401.555236E-824.38139536
Produced by FastQC (version 0.11.5)