FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890594
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78870.4171704765803763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC73360.38802619705764435No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCA61300.32423672136905124No Hit
GTGTAGCAACTGGCCTTCTTTCGGCGGAGGGACCAAGGTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTT61200.32370778707644265No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55130.2916014755151026No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48580.25695627934924153No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGA47680.25219587071576444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46360.2452139380533314No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT44970.2378617513860723No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43330.22918722898729182No Hit
GTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGC43280.22892276184098753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42910.22696570495833582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42660.22564336922681444No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42540.22500864807568416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41770.2209358540225982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41280.2183440759888162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40710.2153291505209474No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTA39910.21109767618007882No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCT39450.20866457843407943No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39130.20697198869773203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37520.19845614658673413No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCCTTCTTTCGGCGGAGGGAC36930.19533543426034355No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC36830.194806499967735No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT36680.19401309852882218No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCACCTTGGTCC35170.1860261907104328No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35150.1859204038519111No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA34810.18412202725704196No Hit
GTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATG32720.17306730054152292No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTGG32540.1721152188148275No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32460.17169207138074066No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31170.16486881900609013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30690.162329934401569No Hit
GTTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT30430.16095470524078676No Hit
GGTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT29770.15746373890957022No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29210.15450170687096226No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29200.1544488134417014No Hit
ACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT28910.15291490399313656No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTTCTACTGGGGCTGGATCCG27990.14804870850113774No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA27950.14783713478409433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27520.14556271732587747No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC26200.1385807846634444No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25600.135407178907793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25530.135036924902967No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25210.1333443351666196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25210.1333443351666196No Hit
CTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAATTGTGTTGACACAGTC25000.1322335731521416No Hit
CTGTTGGTACCAGGCTAAGTTGTCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA24780.13106991770840276No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT24440.12927154111353364No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24410.12911286082575107No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24150.1277376316649688No Hit
GACCAAGGTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT23880.12630950907492566No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23680.12525164048970852No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23500.1242995587630131No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23170.12255407559740483No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22960.12144331358292684No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22820.12070280557327484No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22720.12017387128066628No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCACCTTGGTCCCTCCGCCGAAAGAA22700.12006808442214457No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22590.11948625670027514No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21670.11462006120827634No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21630.1144084874912329No Hit
CAATAATACACAGCCGTGTCTGCGTCGGTCGCATATCTCAACTTCACGGA21630.1144084874912329No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21440.11340351233527664No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21410.11324483204749407No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGA21400.1131919386182332No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21360.11298036490118978No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21350.11292747147192894No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21300.11266300432562465No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21260.11245143060858122No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21250.11239853717932036No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21200.11213407003301608No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20600.1089604642773647No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20450.10816706283845183No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20400.10790259569214754No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20340.1075852351165824No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20330.10753234168732154No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20230.10700340739471298No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19910.10531081765836557No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19860.10504635051206128No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG19690.10414716221462672No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC19660.10398848192684415No Hit
GAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATC19610.10372401478053989No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19480.10303640020014873No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAATTGTGTTGACACAGTCTC19400.10261325276606188No Hit
GCCTGGGAGTCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGTTGTCGCTA19270.10192563818567076No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19190.1015024907515839No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19180.10144959732232303No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19130.10118513017601877No Hit
ATAATACACAGCCGTGTCTGCGTCGGTCGCATATCTCAACTTCACGGAGA19060.10081487617119277No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19050.1007619827419319No Hit
ACCTTGGTCCCTCCGCCGAAAGAAGGCCAGTTGCTACACTGCTGACAGTA18970.10033883530784504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.043.3667341
CGCTAAT3950.042.46994432
CGTATGC3950.042.00177441
TCGTATG4100.040.41523440
CGTTCAA602.9290095E-740.21993339
AGCGTTC602.9835792E-740.12542337
ACTCGCT4300.039.7258629
ATCTCGT3900.039.68448337
AATCTCG4050.039.02326236
GCTAATC4200.037.52048533
CGAGACA4550.037.45668423
ACGAGAC4550.037.4517322
GTGGTAT4550.037.44381
ACACTCG4650.036.69194427
GCGGCAT901.3145246E-436.60204770
GCCGTCT4500.036.3655746
ATGCCGT4600.036.23105244
TATGCCG4500.036.2148143
CTTATAC5250.035.6964261
GTATCAA43250.035.5313571
Produced by FastQC (version 0.11.5)