FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890594
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78720.4163770751414635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64490.3411097253032645No Hit
GTGTAGCAACTGGCCTTCTTTCGGCGGAGGGACCAAGGTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTT60070.31773082956996584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57170.3023917350843174No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCA55230.29213040980771127No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47120.2492338386771565No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGA46970.24844043723824366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45390.2400832754150283No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT44530.23553444049859462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44240.23400053105002977No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44100.2332600230403778No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43560.23040377786029154No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTA41770.2209358540225982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39930.21120346303860055No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39840.21072742217525284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39320.2079769638536883No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCT39010.20633726754660178No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38840.20543807924916718No Hit
GTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGC38570.20400995665912405No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36780.19454203282143073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36410.19258497593877905No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCCTTCTTTCGGCGGAGGGAC35890.18983451761721448No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC35450.1875072067297368No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT35380.1871369527249108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCACCTTGGTCC34960.18491542869595481No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34770.18391045353999855No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33280.1760293325801309No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33280.1760293325801309No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33170.1754475048582615No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA32630.17259125967817524No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTGG32410.17142760423443637No Hit
GTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATG31180.164921712435351No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31120.16460435185978586No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30510.16137785267487362No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30290.16021419723113475No Hit
GTTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT30080.15910343521665676No Hit
GGTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT29990.15862739435330905No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA28500.15074627339344143No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27890.1475197742085292No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTTCTACTGGGGCTGGATCCG27470.1452982501795732No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27050.1430767261506172No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26770.14159571013131322No Hit
CTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAATTGTGTTGACACAGTC26640.1409080955509221No Hit
ACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT26620.14080230869240037No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC25430.13450799061035845No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24960.13202199943509818No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24930.13186331914731558No Hit
CTGTTGGTACCAGGCTAAGTTGTCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA24550.12985336883540305No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24340.12874260682092506No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23900.12641529593344739No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23140.12239539530962226No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23100.12218382159257883No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22870.12096727271957913No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22730.12022676470992714No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22390.11842838811505801No Hit
GACCAAGGTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT22280.1178465603931886No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22050.1166300115201889No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22030.11652422466166719No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCACCTTGGTCCCTCCGCCGAAAGAA21820.1154134626471892No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21610.1143027006327112No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT21590.11419691377418947No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21540.11393244662788521No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21100.11160513574040752No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20710.1095422919992341No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20370.10774391540436497No Hit
CAATAATACACAGCCGTGTCTGCGTCGGTCGCATATCTCAACTTCACGGA20340.1075852351165824No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20210.10689762053619127No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19900.10525792422910471No Hit
GCCTGGGAGTCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGTTGTCGCTA19870.10509924394132214No Hit
GAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGGCTCCTCATCTATGATGCATC19860.10504635051206128No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19850.10499345708280045No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19820.10483477679501785No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19770.10457030964871358No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAACTTAGCCTGGTACCAACAGAGACCTGGCCAGA19690.10414716221462672No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19670.10404137535610501No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC19640.10388269506832246No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19360.10240167904901845No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19190.1015024907515839No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19180.10144959732232303No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19160.10134381046380132No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18960.1002859418785842No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG18930.10012726159080163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.043.6546331
TACGTAT1004.4447606E-640.53145670
GTGGTAT4800.040.4139441
GTATACC451.0855489E-437.814221
CGTGGCA451.0902939E-437.78621310
GTATCAA46200.036.0217251
TGGTATC5650.033.7146642
TATCAAC50650.032.6389432
CCTAACG851.3688805E-732.0147672
ATCAACG53350.030.920083
TCAACGC53800.030.6582134
AACGCAG54100.030.3624786
CAACGCA54450.030.3546855
CTAACGC902.2617678E-730.232973
GCCTAAT900.00512731630.02330470
GACAGGT3700.029.4337671
CGCAGAG56600.028.9612948
TGTTAGA23450.028.80700770
CTAATGG1903.85573E-728.4431370
GCAGAGT58350.028.2085259
Produced by FastQC (version 0.11.5)