FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739841
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG34570.4672625604690737No Hit
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT29390.397247516696155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27870.37670256176664985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27040.3654839350617227No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23010.3110127716631006No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT22810.3083094881197446No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT21670.2929007719226158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20170.2726261453474463No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19290.26073169775668015No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18170.2455933099138869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18080.2443768323193767No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17730.23964608611850383No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17280.23356369814595299No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT17070.23072525042542924No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16360.22112859384651568No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15760.21301874321644787No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15640.2113967730904343No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15050.20342208663753428No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG14400.1946364151216275No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14330.1936902658814529No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA14170.19152763904676817No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA13750.1858507436057207No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13370.18071450487334442No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT13270.17936286310166644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13260.17922769892449864No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG12960.17517277360946473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12790.1728749825976122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12570.16990137069992067No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT12130.16395414690453758No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG12020.16246734095569185No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA11990.16206184842418844No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT11580.15652011716030875No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11550.15611462462880538No Hit
GGACTGAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTT11400.1540871619712884No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11370.15368166943978503No Hit
GTAATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATAGA11230.15178937095943587No Hit
CTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC11080.14976190830191893No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCC10800.14597731134122063No Hit
GGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGA10700.14462566956954265No Hit
CTCCTGTGGTGCCTCTGGATTCACTTTTAGCGCCTATGCCATGACCTGGGTCCGCCAGGCTCCCGGGAAGGGG10660.14408501286087147No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG10650.14394984868370367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10560.14273337108919348No Hit
GGACTCCACTTTCCAAAGTGGAGGCATCAAAGATCAGGAGCTTAGGAGCTTTCCCCGGTCTGTGTTGATACCAGG10480.14165205767185113No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10380.14030041590017314No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10300.13921910248283076No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10270.13881360995132738No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCGCTGCCG9920.1340828637504545No Hit
GCCTTATATTACTGTGCGAAAGGCGAGAGATCATCTATAGAGACTGCCATGGGTTACTACTTTGACTTTTGGGGC9900.1338125353961189No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9880.1335422070417833No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9790.13232572944727314No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA9750.13178507273860193No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTC9570.1293521175495816No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9570.1293521175495816No Hit
CCCAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9440.12759498324640023No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCC9350.12637850565189007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9330.12610817729755447No Hit
CAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCAT9330.12610817729755447No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9280.1254323564117155No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9210.12448620717154092No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGACGACGGAGACTGGGTCAA9160.12381038628570193No Hit
GAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9060.12245874451402396No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8870.11989062514783581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8870.11989062514783581No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8830.11934996843916462No Hit
GGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAG8800.11894447590766123No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8720.11786316249031888No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8670.11718734160447988No Hit
CATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAATCTTCA8650.1169170132501443No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8630.11664668489580869No Hit
ACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCTCCACTTTGGAAAGTGGAGTCCCATCGAGGTT8580.11597086400996971No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8540.11543020730129852No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8520.11515987894696292No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8500.11488955059262733No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8450.11421372970678834No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8440.11407856552962056No Hit
GCACCTGGGTCCCTCCGCCGAAAGTGAGCCCATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAAT8430.11394340135245276No Hit
GGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCTC8400.11353790882094937No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8270.11178077451776802No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8150.11015880439175445No Hit
GTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCA8120.10975331186025106No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8110.10961814768308326No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8070.10907749097441206No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8060.10894232679724428No Hit
GTCTCCGACAGATGCAGACAGGGACGACGGAGACTGGGTCAACTGGATGACACATCTGGCACCTGGGAGCCAGAG7970.10772584920273409No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7910.10691486413972733No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7800.10542805819088155No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCC7760.10488740148221036No Hit
GTATGTGGTTCCACCAGACCCAGTAATGGTCGAGACCCATTCCAGCCCCTTCCCGGGAGCCTGGCGGACCCAGGT7690.10394125224203578No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7630.10313026717902901No Hit
GTGTCATCCAGTTGACCCAGTCTCCGTCGTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGG7580.10245444629319002No Hit
CAGTAATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATA7560.10218411793885443No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7480.10110280452151205No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7460.10083247616717646No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7460.10083247616717646No Hit
ATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATAGAGGG7410.10015665528133746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)