FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739841
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG33240.44928572490575674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26000.3514268606362718No Hit
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT23370.3158786820411413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23150.3129050701434497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22010.2974963539463209No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20830.2815469810405209No Hit
GAGTGAAGTCTGTCCCAGATCCGCTGCCGCTGAACCTCGATGGGACTCCA19370.26181301117402256No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT18140.24518781738238354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17820.240862563713014No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT17420.23545599662630215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16340.22085826549218004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16120.21788465359448855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16110.21774948941732075No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT15930.21531653422830038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15930.21531653422830038No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15820.21382972827945465No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15470.20909898207858174No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15320.20707151942106478No Hit
GTCCCAGATCCGCTGCCGCTGAACCTCGATGGGACTCCACTTTCCAAAGT13710.1853100868970495No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG13390.18098483322768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13390.18098483322768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13310.17990351981033761No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCGCTGCCG12950.17503760943229696No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12590.17017169905425625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12540.16949587816841727No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12360.16706292297939693No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG12100.1635486543730342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12090.1634134901958664No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11880.16057504247534268No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT11610.15692560969181216No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA11370.15368166943978503No Hit
GGACTGAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTT11240.15192453513660367No Hit
CTCCTGTGGTGCCTCTGGATTCACTTTTAGCGCCTATGCCATGACCTGGGTCCGCCAGGCTCCCGGGAAGGGG11230.15178937095943587No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA10930.14773444564440197No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10810.1461124755183884No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10800.14597731134122063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10690.14449050539237485No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT10680.14435534121520704No Hit
GGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGA10610.14340919197503246No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT10060.13597516223080364No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10050.13583999805363584No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA9990.13502901299062905No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9980.13489384881346125No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG9770.13205540109293754No Hit
GGACTCCACTTTCCAAAGTGGAGGCATCAAAGATCAGGAGCTTAGGAGCTTTCCCCGGTCTGTGTTGATACCAGG9670.13070375932125955No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9590.1296224459039172No Hit
GCCTTATATTACTGTGCGAAAGGCGAGAGATCATCTATAGAGACTGCCATGGGTTACTACTTTGACTTTTGGGGC9500.128405968309407No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCC9430.12745981906923243No Hit
CTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC9190.12421587881720532No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA9140.12354005793136634No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9030.12205325198252058No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8900.1202961176793392No Hit
CATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAATCTTCA8700.11759283413598327No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8690.11745766995881549No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8610.1163763565414731No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTCTCCGTCGTCC8580.11597086400996971No Hit
GGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAG8540.11543020730129852No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGACGACGGAGACTGGGTCAA8530.11529504312413072No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8490.11475438641545953No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8480.11461922223829175No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8450.11421372970678834No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8400.11353790882094937No Hit
GTAATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATAGA8280.1119159386949358No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8240.1113752819862646No Hit
GCACCTGGGTCCCTCCGCCGAAAGTGAGCCCATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAAT8230.11124011780909683No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8220.11110495363192902No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8110.10961814768308326No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8090.10934781932874767No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG7950.10745552084839852No Hit
GAACAGAGAGAGCTCGCCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7930.1071851924940629No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCGCTGCCG7930.1071851924940629No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7860.10623904325388833No Hit
CAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCAT7750.10475223730504257No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7720.10434674477353917No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGTTGACCCAGTC7710.1042115805963714No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7650.10340059553336459No Hit
GTCTCCGACAGATGCAGACAGGGACGACGGAGACTGGGTCAACTGGATGACACATCTGGCACCTGGGAGCCAGAG7610.10285993882469342No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7560.10218411793885443No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7540.10191378958451884No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7450.10069731199000866No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT7420.10029181945850527No Hit
CCCAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT7400.10002149110416969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG200.006890596452.61597454
TAACCGT200.006917149352.56482753
GTAACGT200.0073842951.7032938
GTGTAAA551.5873775E-743.4775281
GTGGTAT1700.036.170381
TTAGGGA400.00270201234.21881528
ACGGGAT501.9829643E-434.128583
GAGTTTA1600.032.0258561
TCAACGC14700.031.1040924
ATCAACG14750.031.0049443
GGATATC450.004850389430.3652571
CGCAGTG450.004877747530.3303614
CAACGCA15000.030.2524875
AACGCAG15200.029.8544256
AGTTTAT1750.029.2629572
GACAGGT1700.028.1325171
CGCAGAG16150.028.0982848
CCTAACC500.007255626427.96274451
AGAGTAC16050.027.84630811
GCAGAGT16350.027.756459
Produced by FastQC (version 0.11.5)