FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777881
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGACTCAAGAAGAAGTGGTTCTTGGACGTGTCGACGGA63610.3577854760807951No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59140.3326431859050184No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51860.29169556342634856No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC49830.2802774763890271No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45880.25806001639029835No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42550.23932985391035733No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41900.2356738161890475No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC38740.21789984819006444No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37700.2120501878359688No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37250.20951908479813888No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGCTTGTAAATGAGCTGCAATAATAAT35580.20012588019108138No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35460.19945091938099344No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35110.19748228368490356No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATTT33520.18853905295123802No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT33150.18645792378680012No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGCTTGTAAATGAGCTG32190.1810582373060964No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT31860.1792020950783545No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31460.17695222571139463No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29940.16840272211694707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29790.16755902110433712No Hit
GTGTATTACTGTGCGAGACTCCCTGATGGGAGCCCCGCTGACTACTGGGGCCGGGGAACCCTGGTCACCGTCTC29450.16564663214242123No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATATTTACCACTGGGGCTGGATCCG29440.16559038540824722No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGC29020.16322802257293936No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCA28440.15996571199084753No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC27970.15732211548466968No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27950.15720962201632166No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATT27720.15591594713031975No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26940.15152870186474798No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGACTCAAGAA26370.14832263801683013No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25280.14219174399186446No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24910.14011061482742657No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24410.13729827811872672No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT24220.1362295901694208No Hit
GAATTACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT24010.13504840875176685No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23840.13409221427080892No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23730.13347350019489493No Hit
GGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATAATGTTACTAAT23700.13330475999237293No Hit
GATTATTATTGCAGCTCATTTACAAGCAGAATTACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT22950.12908625492932316No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22930.12897376146097517No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22890.12874877452427919No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22020.12385530865114144No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21050.11839937543626373No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20820.1171057005502618No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19880.11181850753790609No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAATATTTACCACTGGG19840.1115935206012101No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19820.11148102713286209No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19680.11069357285442614No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19600.11024359898103417No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19590.11018735224686017No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19540.10990611857599017No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTACAAGCAGAATTACTTATGT19230.10816246981659627No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18900.10630632758885437No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18840.1059688471838104No Hit
GCCTATTACAGTGGGATCACCTACTACAATCCGTCCCTCAAGAGTCGAGC18840.1059688471838104No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18430.10366273108267651No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18370.10332525067763253No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18230.10253779639919658No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17780.10000669336136671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG150.002472378868.1642762
AGGGGGG3850.054.8882270
GGTATCA17300.046.1034281
CGTATGC3900.043.40226741
TCGTATG3900.043.3367840
GCCGTCT4350.039.95827546
ATCTCGT4100.039.3877437
TCTCGTA4050.039.06883238
CGAGACG4300.038.8377823
ATGCCGT4350.038.25567244
CTACATC4250.037.86657733
GTATGCC4550.037.25611542
TGCCGTC4700.036.9201645
ACGAGAC4550.036.70177522
CATCTCG4400.036.66374236
GTATCAA43150.036.4942671
CGACGGA14350.036.04803569
CACGAGA4650.035.91248721
TATACAC4650.035.1815573
GCTACAT4900.034.21301732
Produced by FastQC (version 0.11.5)