FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777881
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGACTCAAGAAGAAGTGGTTCTTGGACGTGTCGACGGA60410.33978652114511604No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC56700.3189189827665631No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50340.28314605983190105No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA49120.2762839582626734No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC42500.23904862023948734No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41770.23494260864478558No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40950.2303303764425178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39590.2226808205948542No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37830.21278139538023072No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37600.21148772049422881No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36680.2063130209502211No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGCTTGTAAATGAGCTGCAATAATAAT32400.18223941872375035No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32160.18088949710357444No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31760.17863962773661454No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31640.17796466692652657No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31480.17706471917974262No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31210.1755460573570447No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT31190.17543356388869671No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATTT31030.17453361614191276No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30060.16907768292703504No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGCTTGTAAATGAGCTG29970.16857146231946907No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATATTTACCACTGGGGCTGGATCCG29260.16457794419311528No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29070.16350925624380933No Hit
GTGTATTACTGTGCGAGACTCCCTGATGGGAGCCCCGCTGACTACTGGGGCCGGGGAACCCTGGTCACCGTCTC29030.16328426930711334No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28900.1625530617628514No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATT27410.15417229837092583No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC27390.15405980490257784No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCA27170.15282237675074992No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTAATTCTGC25860.1454540545739563No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24870.13988562789073059No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24870.13988562789073059No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGACTCAAGAA24550.1380857323971627No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24520.1379169921946407No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24380.13712953791620475No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23730.13347350019489493No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23460.13195483837219701No Hit
GAATTACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT23220.1306049167520211No Hit
GGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATAATGTTACTAAT22560.12689263229653727No Hit
GATTATTATTGCAGCTCATTTACAAGCAGAATTACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT22070.12413654232201142No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21990.12368656844861944No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT21850.1228991141701835No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21430.12053675133487562No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21160.1190180895121777No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21070.11851186890461173No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20730.11659947994269583No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20410.11479958444912791No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20190.11356215629729999No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19760.11114354672781812No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTACAAGCAGAATTACTTATGT19370.10894992409503222No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC18880.10619383412050638No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18810.1058001069812884No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18790.1056876135129404No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAATATTTACCACTGGG18440.10371897781685051No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18440.10371897781685051No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18320.10304401700676255No Hit
CCCCTGAGGGCCGATTAGTAACATTATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGCTGGTACCAGGAGA17960.10101913457649864No Hit
GCCTATTACAGTGGGATCACCTACTACAATCCGTCCCTCAAGAGTCGAGC17950.10096288784232466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC306.731981E-445.4030953
GGTATCA20350.041.030121
ATAGAAC350.001437393538.916943
GTATCAA46850.036.5170141
CCTAACG400.00276348734.0647772
TACCGGA400.00276348734.0647772
TAACGCC400.002770356134.047534
CGACGGA15500.033.8386269
GTGGTAT6050.032.6718521
TATCAAC53550.031.8065152
AATGGTA6150.031.72176670
ATCAACG54400.031.1728973
TGGTATC6600.030.9679782
CGCAAAT553.510627E-430.96623623
TCAACGC54850.030.9128024
CAACGCA56100.030.2240125
AACGCAG56450.030.0366176
CGCAGAG60100.028.0991298
ACGCAGA60300.028.005937
CGCGCAA500.00823713327.24722521
Produced by FastQC (version 0.11.5)