FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151668
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT88540.4114947101504507No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA63420.29474807451707236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61410.2854064846435416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61070.2838263152121982No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTCCCTCCCTTTCGGCGGAGGGAC60930.2831756572110567No Hit
GTGTAGCAACTCCCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT60370.28057302520649097No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT56970.264771330893056No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54580.2536636693021414No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG53260.24752889386280785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52510.2440432259995501No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50700.2356311475562215No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT46070.21411295794704388No Hit
GTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44680.20765285350713958No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44530.20695571993448805No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT44270.20574735507522535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44020.2045854657874728No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA42460.19733527663189673No Hit
GCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGCAGTGTAGCAAC41880.1946396934843108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39710.18455449446661845No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGCAGTGTAGCAACTCC38660.17967455945805766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37900.17614241602328984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37200.17288912601758263No Hit
GTAATACACGGCGGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA37110.1724708458739917No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC37100.17242437030248162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36870.17135543215774923No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36570.16996116501244615No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35220.16368696285858228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34610.16085195299646599No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34050.15824932099190026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33890.1575057118477386No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA33410.15527488441525367No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT32950.15313700812578893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32830.1525793012676677No Hit
TTGCTACACTGCTGACAGTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC32520.1511385585508545No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC31520.14649100139984422No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30940.14379541825225825No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATG30880.14351656482319763No Hit
GTGTATTACTGTGCGAGAGCCTATTACTATGATAGTACTGGTTATTCGCCCCTCGCCTACTGGGGCCAGGGAACC30780.14305180910809662No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT29430.13677760695423272No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29280.13608047338158116No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG28560.13273423223285374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28540.13264128108983356No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC27980.1300386490852678No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27700.12873733308298493No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26880.1249263362191565No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26850.12478690950462619No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25840.12009287678210578No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC25040.11637483106129758No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24780.11516646620203488No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT24730.11493408834448438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24650.11456228377240356No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG24560.11414400362881262No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24330.11307506548408025No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATGCATC24300.11293563876954996No Hit
CTCCCAGGCTCCTCATCTTTGATGCATCCAACAGGGCCACTGGCATCCCA24080.1119131761963277No Hit
GTTGCTACACTGCTGACAGTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT23590.10963587319233264No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG23320.10838103276155986No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23000.10689381447323658No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22930.10656848547266586No Hit
GAGTTGCTACACTGCTGACAGTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT22840.10615020532907495No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22790.10591782747152441No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22340.10382642675356979No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC22250.10340814660997887No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22050.1024786351797768No Hit
GTAGCAACTCCCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA21810.10136322146353434No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC21630.10052666117635249No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGG21560.10020133217578175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAA14100.041.9102370
CGTAAGG350.001419771739.015112
GGTATCA15950.038.5518881
GTATCAA39100.038.092861
CGGACGA850.00299696633.5044270
TATCAAC44700.032.7635272
ATCAACG45750.032.1436273
TCAACGC45550.031.907264
CAACGCA46350.031.5037545
AACGCAG47150.030.824516
AATGGTA9050.029.89482970
ACGCAGA49500.029.3611247
ATACGTA1052.6006092E-829.24297111
CGCAGAG50250.028.9907938
AGAGTAC51750.028.41418511
TACGTAC1104.0881787E-827.91244912
GTCTACT12800.027.78406167
TCTACTG13550.027.68135368
GCAGAGT52650.027.6692739
GAGTTTA6200.026.9984131
Produced by FastQC (version 0.11.5)