FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108361
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45350.40916271864491804No Hit
GAGTTACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT41450.37397562707457227No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37450.33788630238703815No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAACTGTC32570.29385732626824657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31420.28348164542058046No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29120.26273028372524837No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATGAGCAC29090.26245961379009186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28580.25785822489243126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27770.2505501366432056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24980.22537783267365055No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24550.22149823026974066No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22830.20597982065410098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22640.2042655777314431No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22310.20128820844472153No Hit
GTCCTGATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTT22280.20101753850956502No Hit
GTTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC22210.20038597532753316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22020.1986717324048753No Hit
GTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGA21960.19813039253456227No Hit
GATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCA21280.1919952073376815No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATCACCCAGTC21280.1919952073376815No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGTCTGCCCAGCTCCATGTAGGCTGTGCTGATGGACCTGTCCCTGGT21200.1912734208439308No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21140.1907320809736178No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21020.1896494012329918No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20510.1850480123353312No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20340.18351421603611098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20020.18062707006110823No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCACT19460.17557456460485346No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19440.1753941179814158No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC18080.1631237475876542No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTC17970.16213129115874703No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17530.15816146544311827No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAA17450.15743967894936758No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGGTAGTGTAACTCTGTTGACAG17330.15635699920874155No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17160.15482320290952134No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17140.15464275628608368No Hit
GTGGATGTACCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTG16740.15103382381733027No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAA16330.14733466803685802No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAACTGTCCCAGATCCACTGCCA16280.14688355147826385No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATGAGCACCTATTTAAATTGGTATCAGCAGAAA16170.14589109504935666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16090.14516930855560597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16060.1448986386204495No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16020.14453774537357414No Hit
GAGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA15830.14282350245091627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15750.14210171595716556No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15520.1400265797876324No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTC15290.13795144361809916No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14920.13461318108450226No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14570.13145536517434303No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14530.13109447192746768No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT14120.12739531614699542No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATCACCCAGTCTCCATCCTCC14040.12667352965324474No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13750.12405705361339851No Hit
ACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGA13720.12378638367824202No Hit
CCCCTAAGGTCCTGATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCA13490.12171124750870879No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13270.11972633465089443No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12700.11458360588292081No Hit
GGACTCCACTTTGCAAAGTGGATGTACCATAGATCAGGACCTTAGGGGCT12640.1140422660126078No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12630.11395204270088896No Hit
GTGTATTACTGTGCGAGACGGGGTCGGGGGGGTGACTACGTCTACTACTT12370.11160623659619925No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12270.11070400347901091No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12100.1091702071797907No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11880.10718529432197632No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11860.10700484769853866No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11820.10664395445166332No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11790.10637328451650681No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11730.1058319446461938No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA11670.10529060477588079No Hit
TCCGAGGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA11580.10447859497041126No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11570.10438837165869244No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11560.1042981483469736No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11480.10357636185322291No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11400.10285457535947223No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11400.10285457535947223No Hit
AGTCAGACCATGAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAA11360.10249368211259688No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11310.10204256555400272No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11290.10186211893056506No Hit
ATGGTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCACTCTCA11210.10114033243681436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)