FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108361
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42610.3844415312339572No Hit
GAGTTACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT39280.354397168431585No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAACTGTC31850.2873612478244904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31490.28411320860261235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30120.27175261489713187No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27910.2518132630072693No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATGAGCAC26820.24197892202991625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25380.228986765142404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25320.22844542527209097No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23840.21509237513770335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22470.2027317814322229No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21860.19722815941737395No Hit
GTTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC21500.19398012019549588No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGTCTGCCCAGCTCCATGTAGGCTGTGCTGATGGACCTGTCCCTGGT21380.19289744045486987No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21220.19145386746736848No Hit
GTCCTGATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTT21090.19028096441502362No Hit
GATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCA19870.1792737203853257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19850.17909327376188805No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19750.1781910406446997No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCACT19270.1738603216821956No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATGAGCACCTATTTAAATTGGTATCAGCAGAAA19080.17214607875953775No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18850.17007094259000452No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18840.16998071927828567No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18720.16889803953765967No Hit
GTACATCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGA18590.16772513648531479No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATCACCCAGTC18520.16709357330328298No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17850.161048611418121No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17690.15960503843061963No Hit
GTGGATGTACCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTG17440.15734945563764874No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTC17370.1567178924556169No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17280.15590588265014738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17090.1541916397274895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16700.15067293057045494No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGGTAGTGTAACTCTGTTGACAG16600.14977069745326657No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16510.14895868764779707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16260.14670310485482618No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAA16250.14661288154310734No Hit
GAGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA16080.14507908524388713No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAACTGTCCCAGATCCACTGCCA15760.1421919392688844No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15650.14119948283997724No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC15490.13975590985247588No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15190.1370492105009108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15080.13605675407200363No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14060.12685397627668243No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATCACCCAGTCTCCATCCTCC14050.1267637529649636No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14040.12667352965324474No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAA14030.1265833063415259No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTC13820.12468861679543036No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13400.12089923770323928No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13390.12080901439152046No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT13210.11918499478058141No Hit
ACACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGA12970.11701963529932938No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12840.11584673224698451No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12710.11467382919463966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12670.1143129359477643No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12630.11395204270088896No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12440.11223779977823109No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12200.11007244029697906No Hit
GGACTCCACTTTGCAAAGTGGATGTACCATAGATCAGGACCTTAGGGGCT11840.10682440107510098No Hit
CCCCTAAGGTCCTGATCTATGGTACATCCACTTTGCAAAGTGGAGTCCCA11830.10673417776338216No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11680.10538082808759962No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11670.10529060477588079No Hit
GTGTATTACTGTGCGAGACGGGGTCGGGGGGGTGACTACGTCTACTACTT11650.10511015815244311No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA11580.10447859497041126No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11450.10330569191806642No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11440.10321546860634757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT200.0079807450.69901722
GTGGTAT3850.041.237311
TTAGACC451.14191105E-437.488754
GTATCAA25350.035.31191
TATCAAC28900.031.1855072
CTAATGG1402.7721871E-531.02518870
ATCAACG29600.030.3257643
TCAACGC30000.029.6910864
AACGCAG30700.029.3437966
CAACGCA30850.028.9823845
AGAGTAC31750.028.36953411
GTATAGA606.134578E-428.149581
CGCAGAG32250.027.8288568
ACGCAGA32450.027.761317
GCAGAGT32650.027.2812449
GTATAGG500.00857361427.0235981
AATGGTA3503.6379788E-1226.888570
GAGCTAG550.00979285826.29640261
TGTTAGA14100.026.1843870
CAATCTT1101.3913875E-424.48359169
Produced by FastQC (version 0.11.5)