FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557355
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56740.3643356845420601No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA41770.2682111657265042No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39950.25652468448105925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38540.2474708720876101No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37940.2436181859627381No Hit
GTTTATTACTGTCAGTACTGTAGGAACTCCCTCCCTTTCGGCGGAGGGAC36840.23655492806713949No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34400.22088733782599343No Hit
GTACTGTAGGAACTCCCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT33910.2177409774906813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32880.2111271996429844No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG32840.21087035390132627No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32190.20669661059938166No Hit
GTCTGGGACAGACTTCACCCTCACCATCAGCAGCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGTA32170.20656818772855257No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT30210.19398274638730412No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCTACTTAGCCTGGTA28280.18158993935229925No Hit
GACTTCACCCTCACCATCAGCAGCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGTACTGTAGGAACTCC27430.17613196734206393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26700.17144453255680303No Hit
GTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGGGTGAAGTCTGTCCCAGACCCACTGCCA26400.16951818949436703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26240.16849080652773454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25940.16656446346529852No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT25690.1649591775799352No Hit
GCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGTACTGTAGGAAC25660.1647665432736916No Hit
GTGTATTACTGTGCGAGAGACTATTACTATGATAGTAATGGGTATTCGCCCCTCGGCGACTGGGGCCAGGGAACC25650.16470233183827707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24990.1604643771009179No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24320.1561622109281442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24270.15584115375107152No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22850.14672312992220785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22500.1444757296826992No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22370.14364098102231027No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21930.14081567786407082No Hit
GTAGGAACTCCCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA21320.1368987803037843No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20920.13433032288720298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20830.13375241996847217No Hit
GGGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA20710.1329818827434978No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20560.13201871121227982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20260.1300923681498438No Hit
CTACAGTACTGACAGTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCT20190.12964288810194208No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC19570.12566177910624102No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19480.12508387618751024No Hit
GTCAGAGTCTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATG19110.12270805307717252No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACC19040.12225857302927078No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC18680.11994696135434758No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18420.11827746403356974No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18150.11654375527737734No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17990.11551637231074481No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17520.11249843484626178No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17470.11217737766918912No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16960.10890259446304792No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16910.10858153728597526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16810.10793942293182994No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16720.10736152001309913No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTCGGACGTGTCTA16480.10582044556315033No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16430.10549938838607768No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16290.10460042829027422No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16230.10421515967778702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16200.10402252537154341No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG16080.10325198814656901No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15980.1026098737924237No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15880.10196775943827835No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15800.10145406795496209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT200.007168261352.09572244
GGTATCA14950.043.033861
GTATCAA33750.033.008061
AATGGTA5650.028.6186470
TATCAAC38750.028.5604212
ATCAACG39100.028.2938563
TCAACGC39550.028.0547684
TGTTAGA15850.027.8225470
CAACGCA40000.027.7391515
AACGCAG40700.027.4282956
GGTACCG500.00851458427.0625886
GAGCTAG500.00851458427.0625886
ACGCAGA43050.026.0096287
CGCAGAG43400.025.7219288
TACTGAT24650.025.34409370
AGAGTAC43750.025.28094111
GACAGGT2950.025.2521611
CGGGATA3500.025.14488817
TGTCTAC13450.024.9209766
ACGAACC851.2563399E-424.85653952
Produced by FastQC (version 0.11.5)