FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528002
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCATGTCCTCAACTCTCAGGCTGTTCATTTGCAGATACA17350.3285972401619691No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16600.31439274851231624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15040.28484740588103835No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14170.268370195567441No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13970.2645823311275336No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13520.2560596361377419No Hit
GCCTTGGGCTGACTCACGACGGTCAGCTTGGTCCCTCCGCCGAATGACAC12870.2437490767080428No Hit
GTATATTACTGTGCGAAGGGGTCCGGTTCGGAAATCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCC12270.2323854833883205No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11630.22026431718061676No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11420.21628705951871394No Hit
GATTATTACTGCAGCTCGTATGCAGGCCGCAGGGTGTCATTCGGCGGAGGGACCAAGCTGACCGTCGTGAGTCAG10860.20568103908697316No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCACGACGGTCAGCTTGGTCCCTC10270.19450683898924626No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10050.19034018810534808No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9700.1837114253355101No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9410.17821902189764433No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT9380.1776508422316582No Hit
CTCCTGTATAGTGTCCGGAATCAGCTTCAATTACTACGACATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGG9360.17727205578766747No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9350.1770826625656721No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9140.1731054049037693No Hit
CCTGTATAGTGTCCGGAATCAGCTTCAATTACTACGACATGCACTGGGTC8270.15662819459017202No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGC8260.15643880136817664No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7810.14791610637838493No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7680.14545399449244512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7610.1441282419384775No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7460.14128734360854694No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7170.13579494017068117No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7140.13522676050469506No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7130.1350373672826997No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7040.13333282828474133No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6940.13143889606478762No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCA6900.13068132317680614No Hit
CCCCTGAGGGCCGCTTAATGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGATGTTGGTACCAGGAGA6850.1297343570668293No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6830.12935557062283856No Hit
GTGTCATTCGGCGGAGGGACCAAGCTGACCGTCGTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG6570.12443134685095888No Hit
GTAATAATCAGCCCCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG6460.12234802140900981No Hit
TCTCTGGGCTCCAGGCTGAGGATGGGGCTGATTATTACTGCAGCTCGTAT6410.12140105529903295No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCATTAAG6380.12083287563304683No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6370.12064348241105148No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6210.11761319085912554No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6160.11666622474914867No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5940.11249957386525052No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5850.11079503486729216No Hit
GAATGACACCCTGCGGCCTGCATACGAGCTGCAGTAATAATCAGCCCCATCCTCAGCCTGGAGCCCAGAGACGGT5840.1106056416452968No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC5810.11003746197931069No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5800.10984806875731531No Hit
GGATGGGGCTGATTATTACTGCAGCTCGTATGCAGGCCGCAGGGTGTCATTCGGCGGAGGGACCAAGCTGACCGT5710.10814352975935697No Hit
GTTTGGGGGCTTTGCCTGGGTGATGTTGGTACCAGGAGACATAGTTATAA5600.10606020431740788No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5590.10587081109541252No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5460.1034086992094727No Hit
GCCCTCAGGGGTCCCCGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG5440.10302991276548196No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5420.1026511263214912No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT5400.10227233987750046No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5380.10189355343350971No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5330.10094658732353286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG4700.052.66512770
CAACTAT200.00709576352.22488446
CAACGTT250.00710363252.2083869
GCTATAT306.864981E-445.21726619
CCTAAGT405.0285307E-543.0808440
GGTATCA5350.041.8603251
AACCCGA350.001333555639.50990341
GTATCAA12750.038.3234671
AATATAC4100.034.7400933
AGCATCC5300.034.47723869
GGTACCT400.002822880633.9129519
TAGGGGT502.0573923E-433.912954
ATACGTA400.002825503733.90652511
AATACGT400.002825503733.90652510
TACGTAC400.002825503733.90652512
CACCGTC4550.033.6966157
GTAATAT4250.033.533031
ATACAGC4400.033.139066
ATATTAC4750.032.1280563
CTCTGGA5300.032.01457669
Produced by FastQC (version 0.11.5)