FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692175
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30280.4374616245891574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25450.36768158341459894No Hit
GCGTAGCACCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21990.3176942247264059No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21950.317116336186658No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21710.3136490049481706No Hit
GTCTGGGACAGACTTCAGTCTCATCATCAGCAGCCTAGAGCCTGAAGATT20840.3010799292086539No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20140.29096687976306573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18550.26799581030808683No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAG17590.2541264853541373No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17170.24805865568678442No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16520.2386679669158811No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGACTGAAGTCTGTC16260.2349116914075198No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16090.23245566511359123No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACCTGGCCGCTCACTTTCGGCGGAGGGA15880.22942175027991477No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15770.22783255679560804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15570.22494311409686857No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT15490.22378733701737277No Hit
TCTATGATGTATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAGT15060.2175750352150829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14960.21613031386571316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14760.21324087116697368No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14740.2129519268970997No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT14150.20442807093581827No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13360.1930147722757973No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13250.1914255787914906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13020.18810271968794018No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12820.1852132769892007No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTA12810.18506880485426375No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12790.1847798605843898No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGACTGAAGTCTGTCCCAGACCCACTGCCA12780.18463538844945282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12260.17712283743273016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11940.17249972911474698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11010.15906382056560842No Hit
GTATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC10680.15429624011268828No Hit
GAGTGTTGACAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTATC10630.1535738794380034No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10490.15155126954888576No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9980.1441831906671001No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9870.14259399718279336No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9730.14057138729367571No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9580.13840430526962114No Hit
GACTTCAGTCTCATCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACCTGG9180.13262541987214216No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9090.1313251706577094No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9080.13118069852277242No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGTCAACACTCTGA9000.13002492144327663No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8960.12944703290352874No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8890.1284357279589699No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8890.1284357279589699No Hit
CATCTATGATGTATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTG8800.12713547874453715No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8640.12482392458554557No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8530.12323473110123885No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8530.12323473110123885No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8510.12294578683136491No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8470.12236789829161701No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTATCCAACAGGGCCACTGGCATCCCAGCCAGGTT8460.12222342615668003No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8440.12193448188680608No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8380.12106764907718423No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8350.12063423267237332No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8330.12034528840249936No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8270.11947845559287752No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8190.11832267851338173No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGACTGAAGTCTGTCCCAGACCCACTGCCA8160.1178892621085708No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8130.1174558457037599No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8050.1163000686242641No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8030.11601112435439015No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7970.11514429154476831No Hit
GTTGGATACATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTT7960.11499981940983134No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7910.11427745873514646No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCAGTCTCATCATCAGCAGCC7730.11167696030628092No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7720.11153248817134395No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC7700.11124354390147No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7620.1100877668219742No Hit
TTATTACTGTCAGCAGCGTAGCACCTGGCCGCTCACTTTCGGCGGAGGGA7580.10950987828222633No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7540.10893198974247843No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7510.1084985733376675No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7460.10777621266298264No Hit
ATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAGTCTCATCATCAG7210.10416440928955827No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7200.1040199371546213No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7080.10228627153537763No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7040.10170838299562973No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7010.1012749665908188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT208.587115E-568.87720539
ATGTCGT150.002392569368.7261537
TATACCG150.00250763567.916856
TCGTATG750.064.4080940
TACGTAT350.005202265556.4842570
CGTATGC1000.055.31249241
CACGAGA1150.053.16383721
GTACGTA354.2943563E-449.73179669
GAGCGAT1100.046.5998332
ATGCCGT1200.046.30146444
CCCACGA1400.043.66398619
TCTCCGA1400.043.65767711
ATCTCCG1450.042.15223710
CGAGCCC1450.042.15223715
GGTATCA6250.041.293441
GTATCAA19100.040.536761
CCGTCTT1400.039.89327647
ACGAGAC1550.039.44698722
GTATGCC1500.039.2547542
CTTATAC1650.039.103641
Produced by FastQC (version 0.11.5)