FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531237
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAA61890.4041830232681159No Hit
GATTATTACTGTGCAGCACGGGATGACAGCCTGAGTGGTGTGGTATTCGG60660.39615030201072726No Hit
GTAATAATCAGACTCATCCTCGGACCGGAGCCCATTGATGGCCAGGGAGG58460.38178283309507277No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52300.3415539201312403No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC49030.3201986367884266No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGTCATCCCGTG46660.3047209543656534No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT38480.2513000926701745No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37590.24548779842702337No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37560.24529187839635536No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT37300.24359390479723256No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34260.22374067502287368No Hit
GTATATTACTGTGCGAAAGATATGTACCATGACTACGGTGGTAACTCCGG34010.2221080081006402No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG33450.21845083419483724No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31740.20728339244676036No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT31640.206630325677867No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30610.19990373795826513No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30320.19800984432847432No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29220.19082610987064705No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGG28460.18586280242705733No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27960.1825974685825904No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC27110.17704640104699665No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26360.17214840028029627No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26110.17051573335806278No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24840.1622217853931168No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24760.1616993319780021No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24650.1609809585322194No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24330.15889114487176056No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23960.15647479782685503No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23730.15497274425840024No Hit
ATACCACACCACTCAGGCTGTCATCCCGTGCTGCACAGTAATAATCAGAC23690.15471151755084286No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23680.15464621087395353No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC23220.15164210373704395No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23180.15138087702948663No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23110.15092373029126124No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23090.15079311693748257No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAATGGGCTCCGGT23040.15046658355303588No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22550.1472665563854583No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22330.14582980949389285No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21850.1426950890032046No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC21750.14204202223431123No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA21620.14119303543474981No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21430.13995220857385238No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTTAGTAGCTATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGG21420.13988690189696304No Hit
GTACATATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAAGAGTGTT21410.1398215952200737No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21030.13733994149827883No Hit
GTATAAGGACCACCACTAATTGCTGAGACCCACTCCAGCCCCTTCCCTGG20840.13609911463738142No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20410.13329092753113986No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20400.13322562085425052No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20340.13283378079291447No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20310.13263786076224648No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20170.13172356728579573No Hit
CTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGTAATTTTGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC20080.1311358071937917No Hit
TCCTTATACTACACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCT19500.12734801993421005No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA19260.12578065968886593No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACGATTCCAAGAACACTCTTTATCTGCAAATGAA19140.12499697956619388No Hit
GGATGACAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18850.12310308593640307No Hit
ATACTACACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACGATTCCAAGAACACTCTTTAT18440.12042551218394018No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18300.11951121870748943No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18280.11938060535371077No Hit
GATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG18050.11787855178525598No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18040.11781324510836665No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17970.11735609837014126No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17690.1155275114172398No Hit
CCATTGACCCTTGGCCCCAGATCTCAAAACCACTTCCGGAGTTACCACCG17630.11513567135590375No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGC17500.11428668455634235No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC17100.11167441748076881No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT17010.11108665738876478No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC16900.11036828394298205No Hit
GATATGTACCATGACTACGGTGGTAACTCCGGAAGTGGTTTTGAGATCTGGGGCCAAGGGTCAATGGTCACCGT16790.10964991049719933No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG16670.10886623037452726No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16660.10880092369763793No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16620.10853969699008056No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16520.10788663022118718No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16230.10599273659139637No Hit
CCCTGAAGAGACGGTGACCATTGACCCTTGGCCCCAGATCTCAAAACCAC16130.10533966982250298No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15970.10429476299227357No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAATGGGCTCCGGTCCGAGGATG15910.10390292293093754No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15820.1033151628389335No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15740.10279270942381878No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15730.10272740274692946No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15720.1026620960700401No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15620.10200902930114673No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15340.10018044234824525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT150.002416070368.5610358
TCGTATG501.1150405E-955.21304340
GCGTAAA200.007466998451.56052833
GGCGTAA200.0074766751.54364832
TGTCTAT200.007492164651.51666631
TGGTATC2800.047.755662
GGTATCA10450.046.927041
CTACGCT1250.046.6550228
ACGAGAC1350.045.71034222
TCTGGAG15750.043.7753570
CACGAGA1450.042.55790321
CGTATGC702.1676897E-839.47422441
CATCTCG1150.038.90689536
ATCTCGT901.2551027E-1038.25938437
TACGCTG1450.037.8651129
ATACACA1850.037.0648654
GTATCAA25400.036.7230381
TCCGAGC1600.036.42899713
CGAGACT1700.036.30175823
TATACAC1650.035.3239363
Produced by FastQC (version 0.11.5)