FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047182
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGGAGATACA48740.465439627495507No Hit
GTGTATTACTGTGCGAGAGGTGAGGCAGGTCGCGACAACGTAAACTTATT43520.4155915590604116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39550.37768028862222613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38790.37042271544010497No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28980.27674272476035683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25330.2418872746093802No Hit
GTCCCAACCAGGACTCAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG23700.22632169002140984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22640.2161992853200303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22210.21209302680909337No Hit
ATATTACTGTCAACAGTATGATAATTTCCCGTCTTTCGGCGGCGGGACCAAGGTGGACATTAGACGAACTGTGG21910.20922819528983502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21370.20407149855516996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21320.20359402663529358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21170.2021616108756644No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21090.20139765580386215No Hit
GTATGATAATTTCCCGTCTTTCGGCGGCGGGACCAAGGTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTT20730.19795985798075214No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAA20660.19729139729292522No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTC20520.1959544759172713No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20380.19461755454161742No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19970.190702284798631No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19380.18506811614408958No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19090.1822987790088065No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18910.18057988009725148No Hit
GTATTACTGTGCGAGAGGTGAGGCAGGTCGCGACAACGTAAACTTATTACGCTTCTGGGGCCAGGGGACCCTGGT18820.17972043064147397No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18450.17618713843438868No Hit
CCCCTAAACTCCTGATCTACGATGTTTCCAATGTGGAAACAGGGGTCCCA18450.17618713843438868No Hit
AACCAGGACTCAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17740.1694070371721439No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17650.1685475877163664No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG17180.1640593516695283No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17170.16396385728555302No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17050.1628179246778497No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16880.16119452015026994No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGGAGATA16550.15804320547908576No Hit
ACATATTACTGTCAACAGTATGATAATTTCCCGTCTTTCGGCGGCGGGAC16470.15727925040728355No Hit
GCAGAAACCAGGGCAAGCCCCTAAACTCCTGATCTACGATGTTTCCAATGTGGAAACAGGGGTCCCATCAAGGTT15900.15183607052069267No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15610.1490667333854096No Hit
GTTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGAT15520.1482072839296321No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC15500.14801629516168155No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAACCGCTTCCA15470.1477298120097557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15450.14753882324180514No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.14438750857062096No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14920.1424776208911154No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14850.14180916020328846No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14480.13827586799620314No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14460.13808487922825258No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14390.13741641854042563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14310.13665246346862342No Hit
AGTCAGGACATTAACAACTATTTAAATTGGTATCAGCAGAAACCAGGGCA14280.13636598031669758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14190.13550653086092007No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13990.1335966431814145No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAACTATTTAAATTGGTATCAGCAGAAA13720.13101829481408198No Hit
GGACCAAGGTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC13540.12929939590252695No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13260.12662555315121918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13210.12614808123134277No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13150.1255751149274911No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAACCGCTTCCA13130.12538412615954056No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13090.12500214862363945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATGTCCACCTTGGTCC12960.12376072163196082No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12800.12223281148835638No Hit
GTTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTTA12760.12185083395245527No Hit
GTCTGGGGGAGGCTTGGTCAAGCCTGGAGGGTCCCTGAGACTCTCCTGTG12630.12060940696077664No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12620.12051391257680137No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12570.12003644065692497No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12520.11955896873704858No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12450.11889050804922163No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12420.1186040248972958No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12390.11831754174536994No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12330.1177445754415183No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12180.1163121596818891No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12070.11526172145816103No Hit
CTCCAGAGACAACGCCAAGAACTCAGTGTATCTCCAAATGAACAACCTGAGAGCCGAGGACACGGCTGTGTATTA12060.11516622707418576No Hit
GTGTATCTCCAAATGAACAACCTGAGAGCCGAGGACACGGCTGTGTATTA11990.11449776638635882No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTCCCCTGG11930.11392480008250713No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCATCCTCC11730.11201491240300158No Hit
CTACGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAACGCCAAGAACTCAGTGTATCTCCAAATGAA11620.11096447417927352No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11580.11058249664337241No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11340.10829063142796572No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTTC11200.10695371005231183No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11020.1052348111407568No Hit
GTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGCGGTTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTG11020.1052348111407568No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11000.10504382237280624No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10970.10475733922088043No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10790.10303844030932542No Hit
GTCCCAGAACCGCTTCCACTGAACCTTGATGGGACCCCTGTTTCCACATT10790.10303844030932542No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10760.10275195715739957No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10760.10275195715739957No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10710.10227448523752317No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10580.10103305824584456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10580.10103305824584456No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10520.10046009194199289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGT200.007434699751.6159434
CGAGTCG355.09238E-448.04567769
GTGGTAT2150.044.653311
TCTGGAG15150.044.48268570
TCGTATG856.548362E-1140.6604540
GTATTAA603.0548836E-740.0019231
GAATTAG350.001315914939.62080444
CGTATGC901.200533E-1038.42743741
GCCGTTA451.0392188E-438.0934315
CCGTTAA451.039511E-438.0916116
GTATCAA21050.037.626511
ATGCCGT1003.619789E-1034.66820544
ATCTCGT807.129347E-834.4800437
TATACAC1208.367351E-1131.4255773
CGTTAAA553.378949E-431.16735317
ATTCACT1650.031.1658632
ATCAACG25350.030.9691563
TATCAAC25450.030.7127652
GCTTGTA450.00476442830.4776551
GTGCTAG902.1059896E-730.4776551
Produced by FastQC (version 0.11.5)