FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047182
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGGAGATACA45670.4361228516150965No Hit
GTGTATTACTGTGCGAGAGGTGAGGCAGGTCGCGACAACGTAAACTTATT42390.40480069367120525No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39170.37405150203116555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30240.28877501714124193No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28110.26843471335450764No Hit
GTCCCAACCAGGACTCAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG25090.23959540939397353No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24950.23825848801831964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.22135598205469537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22840.2181091729995359No Hit
ATATTACTGTCAACAGTATGATAATTTCCCGTCTTTCGGCGGCGGGACCAAGGTGGACATTAGACGAACTGTGG20740.19805535236472743No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20140.19232568932621072No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19800.18907888027105127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.18793294766334792No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAA19360.184877127376139No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19310.1843996554562626No Hit
GTATGATAATTTCCCGTCTTTCGGCGGCGGGACCAAGGTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTT19260.18392218353638623No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19170.18306273408060872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19080.1822032846248312No Hit
GTATTACTGTGCGAGAGGTGAGGCAGGTCGCGACAACGTAAACTTATTACGCTTCTGGGGCCAGGGGACCCTGGT18910.18057988009725148No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTC18630.17790603734594368No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18600.17761955419401784No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18570.177333071042092No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18010.17198538553947643No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17690.16892956525226752No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17570.1677836326445642No Hit
CCCCTAAACTCCTGATCTACGATGTTTCCAATGTGGAAACAGGGGTCCCA16360.1562288121835555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16310.1557513402636791No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16280.15546485711175326No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16160.1543189245040499No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAACTATTTAAATTGGTATCAGCAGAAA16140.15412793573609937No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16130.15403244135212407No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15970.15250453120851962No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15900.15183607052069267No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15730.15021266599311295No Hit
AACCAGGACTCAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15720.15011717160913768No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAACCGCTTCCA15510.14811178954565682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15490.14792080077770628No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGGAGATA15450.14753882324180514No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15020.14343256473086818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATGTCCACCTTGGTCC14980.14305058719496705No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14240.13598400278079648No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14190.13550653086092007No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC14060.13426510386924145No Hit
ACATATTACTGTCAACAGTATGATAATTTCCCGTCTTTCGGCGGCGGGAC13900.132737193725637No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAACCGCTTCCA13710.1309228004301067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13450.12843994644674947No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13310.12710302507109558No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13190.12595709246339223No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13050.12462017108773833No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13030.12442918231978778No Hit
GTCTGGGGGAGGCTTGGTCAAGCCTGGAGGGTCCCTGAGACTCTCCTGTG13020.1243336879358125No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12990.12404720478388666No Hit
GCAGAAACCAGGGCAAGCCCCTAAACTCCTGATCTACGATGTTTCCAATGTGGAAACAGGGGTCCCATCAAGGTT12910.12328324971208444No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12880.1229967665601586No Hit
GTTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGAT12720.12146885641655414No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12720.12146885641655414No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12650.12080039572872718No Hit
GGACCAAGGTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC12530.11965446312102385No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12450.11889050804922163No Hit
GTGTATCTCCAAATGAACAACCTGAGAGCCGAGGACACGGCTGTGTATTA12420.1186040248972958No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12290.11736259790561716No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12170.11621666529791383No Hit
GTTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTTA12110.11564369899406216No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12060.11516622707418576No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12060.11516622707418576No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCATCCTCC12000.11459326077033409No Hit
AGTCAGGACATTAACAACTATTTAAATTGGTATCAGCAGAAACCAGGGCA11960.11421128323443298No Hit
CTCCAGAGACAACGCCAAGAACTCAGTGTATCTCCAAATGAACAACCTGAGAGCCGAGGACACGGCTGTGTATTA11960.11421128323443298No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTCCCCTGG11770.11239688993890269No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11750.11220590117095212No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11590.11067799102734768No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11570.11048700225939712No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11460.10943656403566905No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11350.108386125811941No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11300.10790865389206461No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11230.10724019320423767No Hit
CCTTGGTCCCGCCGCCGAAAGACGGGAAATTATCATACTGTTGACAGTAA11180.10676272128436128No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11110.10609426059653433No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11110.10609426059653433No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10960.10466184483690513No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10730.10246547400547373No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10650.10170151893367152No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10640.10160602454969622No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10640.10160602454969622No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT10580.10103305824584456No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10550.10074657509391872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTA200.006592486553.20926362
GTATAGA350.00140074839.120471
CGCGGTA801.5679689E-938.99176443
GTGGTAT2400.038.5092121
TCTGGAG11850.038.1113970
ACCCTGG15700.037.52035570
TAACGGC754.0638042E-836.754836
GTATCAA20150.036.0141261
GGACTAG400.002698040834.230411
GACAGGT2050.033.395521
TAGAGGA850.004522833530.80102270
AACGGCC902.006309E-730.6466937
TATCAAC23850.030.2704872
ATCAACG24700.029.3617063
CGGTACC953.0390402E-729.22782545
TCAACGC24850.029.1858674
CAACGCA24950.029.0716675
TTAACGG953.2557182E-728.99881435
AACGCAG25200.028.7832576
TATTAGA1452.5465852E-1128.3164372
Produced by FastQC (version 0.11.5)