FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900988
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA40070.44473400311657874No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGCAACTCTGCCATGAGCTGGGTCCGCCAGTCTCCAGTCAAGGGG29930.3321908837853556No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT29670.3293051627768627No Hit
GTATTATAATACTCCTTTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29300.3251985598032382No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27790.30843918010006793No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26540.29456552140539055No Hit
ATTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA25350.2813577983280577No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24490.2718127211461196No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22780.2528335560518009No Hit
ATATATTACTGTGCGAAGACCCCCGGGGAACACCACTATGGTTCGTCGGG20160.22375436742775706No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAATATT19970.22164557130616613No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19690.21853787175855838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18700.20754993407237388No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18500.2053301486812255No Hit
CCATAGTGGTGTTCCCCGGGGGTCTTCGCACAGTAATATATGGCCGTGTC18470.20499718087255325No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17600.19534111442105778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16320.18113448791770811No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16280.18069053083947845No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16270.180579541569921No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16090.17858173471788746No Hit
CCACTATGGTTCGTCGGGGGGTTATTGGGAGACCAACTGGTTCGAGCCCTGGGGCCAGGGAACCCTGGTCACCGT15620.17336523904868875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15370.1705905073097533No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15000.16648390433612878No Hit
TTATAATACTGCTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC14970.16615093652745652No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14460.16049048378002814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14410.15993553743224107No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14230.15793773058020752No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12900.14317615772907075No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12690.14084538306836494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12520.13895856548588884No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA12390.13751570498164237No Hit
CCATTAACTGCAAGTCCAGCCAGAATATTTTATACAGCTCCAACAATAAG12180.13518493032093656No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12150.1348519625122643No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12010.13329811273846043No Hit
TTATTACTGTCAGCAGTATTATAATACTCCTTTAACTTTTGGCCAGGGGA11940.1325211878515585No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11820.1311893166168695No Hit
CTTTATTACTGTCAGCAGTATTATAATACTCCTTTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11630.12908052049527852No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11390.12641677802590046No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11380.12630578875634305No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10940.12142226089581658No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10880.12075632527847208No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT10840.12031236820024241No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTAACTGCAAGTCCAGCCAGAATATTTTATACAGCTCCAA10820.12009038966112756No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10820.12009038966112756No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC10810.11997940039157014No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTAACTGCAAGTCCAGCCAGAATATTTTATA10800.11986841112201273No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10790.11975742185245532No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT10560.11720466865263465No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10490.11642774376573273No Hit
GTATAAAATATTCTGGCTGGACTTGCAGTTAATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10440.11587279741794564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10370.1150958725310437No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTTAGCAACTCTGCCATGAGCTGGGTCCG10200.11320905494856758No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10170.11287608713989532No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT10060.1116552051747637No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC9920.11010135540095983No Hit
GTATTATAATACTGCTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT9880.10965739832273017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9860.10943541978361532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9850.1093244305140579No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9800.1087694841662708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.1087694841662708No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC9700.1076595914706966No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT9700.1076595914706966No Hit
ATATTACTGTGCGAAGACCCCCGGGGAACACCACTATGGTTCGTCGGGGG9600.10654969877512241No Hit
CTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCGACAACACAT9570.10621673096645017No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.10621673096645017No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGCTCGAACCAGT9450.10488485973176113No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTAACTGCAAGTCCAGC9400.10432991338397404No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9340.10366397776662953No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9200.10211012799282565No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC9190.10199913872326824No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT9170.10177716018415339No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC9090.10088924602769404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC252.5916524E-455.09276636
CGTATGC1000.051.86664241
GACGTAG1150.050.65207726
ACGTAGA1250.046.61285827
GTGGTAT1500.045.6633531
GGTATCA5800.045.466531
AGACGTA1300.044.79516225
CGAGACG1300.044.77775623
TCGTATG1100.043.9661640
ATCTCGT1200.043.06286237
CGTAGAG1400.041.62787628
GTATGCC1250.041.53289442
AACGCTA350.001172123840.56353861
TGGTATC1750.039.1291542
TATACAC1700.038.266013
ACTACAC451.0473638E-438.0422333
GCCGTCT1100.037.8911446
CCGTCTT1200.037.65769647
TGCCGTC1200.037.59412845
ATGCCGT1200.037.5645444
Produced by FastQC (version 0.11.5)