FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900988
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA38350.4256438487527026No Hit
GTATTATAATACTCCTTTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31270.3470634459060498No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGCAACTCTGCCATGAGCTGGGTCCGCCAGTCTCCAGTCAAGGGG28650.31798425728200597No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28030.311102922569446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27120.30100289903972083No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT25580.2839105515278783No Hit
ATTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA22650.2513906955475545No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22390.24850497453906156No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22300.2475060711130448No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21580.23951484370491063No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19740.2190928181063455No Hit
ATATATTACTGTGCGAAGACCCCCGGGGAACACCACTATGGTTCGTCGGG18450.20477520233343843No Hit
TTATAATACTGCTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC17940.19911474958601003No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAATATT17730.19678397492530422No Hit
CCATAGTGGTGTTCCCCGGGGGTCTTCGCACAGTAATATATGGCCGTGTC16830.1867949406651365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16310.1810234986481507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16250.1803575630308062No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16190.17969162741346167No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15630.1734762283182462No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15580.1729212819704591No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15500.17203336781399975No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14950.16592895798834167No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14900.1653740116405546No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14050.15593992372817395No Hit
CCACTATGGTTCGTCGGGGGGTTATTGGGAGACCAACTGGTTCGAGCCCTGGGGCCAGGGAACCCTGGTCACCGT13620.15116738513720493No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13030.1446190182333172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12920.1433981362681856No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12850.14262121138128367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12820.14228824357261138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12330.13684976936429785No Hit
CTTTATTACTGTCAGCAGTATTATAATACTCCTTTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11970.1328541556602308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11840.13141129515598432No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11670.12952447757350818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11610.12885854195616367No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11450.12708271364324497No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11390.12641677802590046No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11350.1259728209476708No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11230.12464094971298176No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11210.1244189711738669No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11120.12342006774785014No Hit
TTATTACTGTCAGCAGTATTATAATACTCCTTTAACTTTTGGCCAGGGGA11090.12308709993917788No Hit
GTATTATAATACTGCTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11080.12297611066962046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11060.12275413213050564No Hit
GTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA11040.12253215359139077No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT10900.12097830381758692No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10810.11997940039157014No Hit
GGGTTCCCTGGCCCCAGGGCTCGAACCAGTTGGTCTCCCAATAACCCCCC10740.11920247550466821No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10630.1179815935395366No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10620.11787060426997917No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10300.11431894764414176No Hit
CCATTAACTGCAAGTCCAGCCAGAATATTTTATACAGCTCCAACAATAAG10260.11387499056591209No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT10250.11376400129635468No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGC10110.1122101515225508No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10110.1122101515225508No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10080.11187718371387853No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10010.1111002588269766No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTTAGCAACTCTGCCATGAGCTGGGTCCG9950.11043432320963209No Hit
CAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA9910.10999036613140241No Hit
GTATAAAATATTCTGGCTGGACTTGCAGTTAATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA9890.10976838759228758No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA9840.10921344124450048No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9460.10499584900131856No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9420.10455189192308888No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC9380.1041079348448592No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT9300.10322002068839985No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTAACTGCAAGTCCAGCCAGAATATTTTATA9290.10310903141884244No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGCTCGAACCAGT9210.10222111726238307No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT9180.10188814945371082No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTAACTGCAAGTCCAGCCAGAATATTTTATACAGCTCCAA9130.10133320310592372No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9080.10077825675813663No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC9060.10055627821902177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC200.00763324751.272421
GGTATCA6400.043.7951931
AAAACCG806.543422E-541.19876570
GTGGTAT1850.040.6484071
TCCCTAG700.001686086237.66744270
GTATCAA15400.037.510991
TGGTATC2050.036.6704862
AATGGTA2650.034.82461570
TCGCACG501.9627319E-434.18920520
CGTACCC400.00272195934.168333
TCTGGAG17300.033.53055670
TATCAAC17700.032.0466542
ATCAACG18250.030.8919183
TGTTAGA6200.030.83262470
GCGACGA450.004605927530.68870239
TAGGTAC450.004845830.371853
AACGCAG18600.030.31236
TCAACGC18600.030.3106174
CAACGCA18700.030.1502045
TCCGAGA5300.029.8496770
Produced by FastQC (version 0.11.5)