FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123313
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49430.44003763866348916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45600.40594206601365784No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41620.37051115762036047No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39570.35226156912632545No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA34700.3089076686551299No Hit
GGCCAAAAGTGTTCGGGGTTCTGTAAGTCTGTTGACAGTAGTAACTTGCT34400.30623699716819797No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34120.3037443704470615No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAA33260.2960884455178565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32900.2928836397335382No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29800.2652867010352413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28950.2577197984889341No Hit
GATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA28570.2543369479388202No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28050.2497077840281382No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27570.24543470964904704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27400.24392132913978565No Hit
GACTTACAGAACCCCGAACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT27230.24240794863052417No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27140.24160674718444458No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26930.23973727714359222No Hit
GTAGTAACTTGCTAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26510.23599833706188744No Hit
CTGTAAGTCTGTTGACAGTAGTAACTTGCTAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC26390.2349300684671147No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26220.23341668795785322No Hit
ACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT25840.23003383740773944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25500.22700707638921655No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24970.22228889009563674No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG24720.22006333052319343No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24410.21730363665336375No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTTCGGGGTT24210.2155231889954091No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23310.2075111745346132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23130.20590877164245405No Hit
AGTTACTACTGTCAACAGACTTACAGAACCCCGAACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA22960.2043953911331926No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22630.20145765249756747No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAACAGACTTACAGAACC21480.19122007846432829No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20950.18650189217074847No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20670.184009265449612No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA20360.1812495715797823No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCG19510.1736826690334751No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19330.1720802661413159No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG19180.17074493039784994No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19130.17029981848336126No Hit
ATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA19130.17029981848336126No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18100.16113051304489487No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18090.16104149066199716No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18040.16059637874750848No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCC18030.16050735636461075No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17960.15988419968432663No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17320.15418676717887178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17200.15311849858409898No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16420.14617475271807592No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16020.14261385740216664No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15680.13958709638364372No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15610.1389639397033596No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG15250.13575913391904126No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAG15080.13424575340977982No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15040.1338896638781889No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAACCAGGGAAAG15000.13353357434659796No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14920.1328213952834161No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14670.1305958357109728No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14360.1278361418411431No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14050.12507644797131343No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13930.12400817937654063No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13860.12338502269625652No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAGAGCTGCTA13830.12311795554756332No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13800.12285088839887014No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13710.12204968695279053No Hit
GTCCCCTACAGATGCAGACAGGGAGGAGGGAGACTGGGTCATCTGGATGT13700.12196066456989282No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAG13530.12044728406063135No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13510.12026923929483589No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13500.12018021691193817No Hit
AGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAACCAGGGAA13400.11928999308296086No Hit
GTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAACAGACTTACAGAACCCCGAACACTTTTGGCCAGGGGA13370.11902292593426765No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13220.11768759019080167No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13120.11679736636182436No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13080.11644127683023342No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13070.1163522544473357No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13010.1158181201499493No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT12880.11466082917227878No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12850.1143937620235856No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12830.11421571725779012No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTA12800.11394865010909694No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAAC12730.11332549342881279No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12530.11154504577085816No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCA12490.11118895623926724No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12410.11047677717608538No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12410.11047677717608538No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12320.1096755757300058No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12170.10834023998653981No Hit
GTTTGGTCCCCTGGCCAAAAGTGTTCGGGGTTCTGTAAGTCTGTTGACAG12140.10807317283784662No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12030.10709392662597156No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG12000.10682685947727838No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT11960.10647076994568744No Hit
ATGCAACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAGAGCTGCTAATGCT11860.10558054611671013No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11850.1054915237338124No Hit
CCCCTAAGCTCCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCA11800.10504641181932374No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11750.10460129990483508No Hit
CTCCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT11730.1044232551390396No Hit
GCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCCCCTACAG11690.10406716560744868No Hit
GAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGTTGCATC11640.10362205369296001No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11520.10255378509818724No Hit
CTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC11450.10193062841790312No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11420.10166356126920992No Hit
GGTTTCTGCTGATACCAATTTAAAGAGCTGCTAATGCTCTGACTTGCCCG11240.10006115837705074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)