FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123313
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48150.4286427736525795No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39130.34834458427882525No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35590.3168306607330281No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35350.31469412354348253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34030.30294316900098195No Hit
GGCCAAAAGTGTTCGGGGTTCTGTAAGTCTGTTGACAGTAGTAACTTGCT31230.27801690178961697No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA30980.27579134221717366No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAA30000.2670671486931959No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29480.26243798478251384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29340.2611916714219456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25580.2277192554523984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25370.22584978541154602No Hit
GACTTACAGAACCCCGAACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT25270.2249595615825687No Hit
GTAGTAACTTGCTAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA25170.22406933775359136No Hit
GATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA25100.22344618107330727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24930.22193280056404582No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24330.2165914575901819No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24040.21400980848614767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24010.21374274133745447No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23860.21240740559398846No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23780.21169522653080663No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23250.20697704023722685No Hit
CTGTAAGTCTGTTGACAGTAGTAACTTGCTAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC23140.2059977940253518No Hit
ACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT22860.20350516730421528No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG22530.20056742866859015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22110.19682848858688537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21880.19478097378023757No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTTCGGGGTT21840.19442488424864665No Hit
AGTTACTACTGTCAACAGACTTACAGAACCCCGAACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA20530.18276295208904375No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20460.18213979540875963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20090.17884596724154356No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAACAGACTTACAGAACC19970.17777769864677076No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19730.1756411614572252No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19700.17537409430853199No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19350.17225831090711136No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA19130.17029981848336126No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA18880.16807425891091796No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18680.16629381125296333No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18570.1653145650410883No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCG18310.16299998308574726No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG18290.1628219383199518No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17150.15267338666961033No Hit
ATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA17030.15160511807483756No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16850.15000271518267838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16790.149468580885292No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT16790.149468580885292No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCC16600.14777715561023508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15900.14154558880739385No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14950.1330884624321093No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT14850.13219823860313198No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14850.13219823860313198No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14840.13210921622023425No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14820.1319311714544388No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14710.13095192524256374No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14540.1294385447333023No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14230.12667885086347258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14170.12614471656608622No Hit
GTCCCCTACAGATGCAGACAGGGAGGAGGGAGACTGGGTCATCTGGATGT13900.12374111222784745No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13890.12365208984494971No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13440.11964608261455177No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13440.11964608261455177No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCAGAAACCAGGGAAAG13420.11946803784875631No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAAC13350.1188448811684722No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAG13330.11866683640267672No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13310.11848879163688125No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13240.11786563495659713No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAGAGCTGCTA13160.11715345589341528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12890.11474985155517652No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12860.11448278440648332No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12680.11288038151432414No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT12600.1121682024511423No Hit
GTCTGCAACCTGAAGATTTAGCAAGTTACTACTGTCAACAGACTTACAGAACCCCGAACACTTTTGGCCAGGGGA12510.1113670010050627No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12460.11092188909057404No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12240.10896339666682393No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12200.10860730713523302No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAGCAAGTTA12200.10860730713523302No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12110.10780610568915341No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12080.10753903854046022No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12030.10709392662597156No Hit
CTCCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT11930.10620370279699423No Hit
GTTTGGTCCCCTGGCCAAAAGTGTTCGGGGTTCTGTAAGTCTGTTGACAG11890.10584761326540332No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11860.10558054611671013No Hit
GGTTTCTGCTGATACCAATTTAAAGAGCTGCTAATGCTCTGACTTGCCCG11830.10531347896801692No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCA11520.10255378509818724No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTAG11450.10193062841790312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25000.038.6244051
GTGGTAT2850.037.113531
TCTATAC656.025821E-736.6813433
TATCAAC30000.032.2647632
ATCAACG31100.031.1041643
TTCGGTC553.4462108E-431.06297728
TCAACGC31300.031.0073324
CAACGCA31550.030.7602625
AACGCAG32200.030.4565816
CGCAGAG33500.029.1730378
ACGCAGA33700.028.99997
AGAGTAC33950.028.68477611
GTGTGTA605.852858E-428.3767939
GTCGTAA500.007330757627.90506248
GCAGAGT35050.027.7857769
GGGTACG500.00816372727.2964021
ATAAACG500.00823324527.2493
GACAGGT2700.026.5381681
AGGTATC907.627772E-626.4920833
CGTACCA907.633051E-626.48972114
Produced by FastQC (version 0.11.5)