FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882988
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAATAGCCGTGTCATCAGTTCTCAGGTTGCTCATTTGAAGATATA35920.40680054542077587No Hit
ATTTACTACTGTGCGACCGACTATGGTTCGGGGAGCAGAAAACAACGTGT29200.3306953208877131No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24060.2724838842656978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23390.26489601217683595No Hit
ATACTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATT21340.24167938862136293No Hit
CTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATTCCGGCAACACAGTATATCTTCAAATGAG20320.23012770275473732No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20140.22808916995474457No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18910.21415919582146076No Hit
GTATAGTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCC18840.21336643306590802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17160.19434012693264235No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAAC17040.1929811050659805No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16390.18561973662156225No Hit
CTCCTGTGCAGCCTCTGGATTCCGCGTCAGTAATACCTTCATCAACTGGGTCCGCCAGGCTCCAGGGAAGGGC16360.18527998115489677No Hit
GGTACATACTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATTCCGGCAACACAGTATATCTT16270.1842607147549004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15520.17576682808826394No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14870.16840545964384568No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14570.1650079049771911No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGTTGATGAAGGTATTAC14470.16387538675497287No Hit
GTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCCCTTCC14270.1616103503105365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13970.1582127956438819No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13890.15730678106610735No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13610.1541357300438964No Hit
GGCCTGGAGTGGGTCTCAATTATTTATAGCGGTGGAGGTACATACTATAC13550.15345621911056548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13180.14926590168835818No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13160.14903939804391453No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12940.1465478579550345No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12670.14349005875504536No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12670.14349005875504536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12590.1425840441772708No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12570.14235754053282718No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12300.13929974133283804No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12210.13828047493284168No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTCGCTATTTTAAAAGGTGGCCA12050.13646844577729256No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12050.13646844577729256No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT11980.13567568302173982No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11790.13352389839952525No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11750.13307089111063797No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11730.1328443874661943No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG11680.13227812835508523No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11650.13193837288841978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11630.13171186924397613No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11510.1303528473773143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11460.1297865882662052No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11430.12944683279953972No Hit
GTCTGTATAGTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGAC11220.12706854453288155No Hit
GCACTGGGCTTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAACACAGA10980.12435050079955785No Hit
GTCTGCATCTGTGGGGGACAGAGTCACCATCACCTGCCGGGCGAGTCGGG10970.12423724897733604No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10940.12389749351067059No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC10910.12355773804400513No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10880.12321798257733967No Hit
CCTGAGAACTGATGACACGGCTATTTACTACTGTGCGACCGACTATGGTTCGGGGAGCAGAAAACAACGTGTCGA10840.1227649752884524No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG10840.1227649752884524No Hit
CTATGACAGAGCCCCGCTCACCTTCGGCGGGGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCAT10820.12253847164400876No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCTGGGTCCCCCGGCCCCAGTAGTCGACACGTTGTTTT10740.1216324570662342No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10510.11902766515513234No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10490.1188011615106887No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCGACTTATTACTGTCACAACTATGACAGAGCC10460.11846140604402325No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10450.11834815422180144No Hit
GTTCTCAGGTTGCTCATTTGAAGATATACTGTGTTGCCGGAATTGTCTCT10250.11608311777736503No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10140.11483734773292502No Hit
GTATATCTTCAAATGAGCAACCTGAGAACTGATGACACGGCTATTTACTA10020.11347832586626318No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT9880.11189280035515771No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9860.11166629671071407No Hit
GTGCATCCACTTTGCAATCAGGGGTCCCGTCTCGATTCAGCGCCAGTGGG9860.11166629671071407No Hit
GTGTGAGGCACAATTGGTGGAGTCGGGGGGAGGCCTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9760.11053377848849588No Hit
GTGGGGGACAGAGTCACCATCACCTGCCGGGCGAGTCGGGGCATCGATAATTATTTGGCCTGGTATCAGCAAAGA9700.10985426755516496No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9660.10940126026627768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.10758923111072857No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCTGGGTCCCCCGG9280.10509769102184853No Hit
CAGTATATCTTCAAATGAGCAACCTGAGAACTGATGACACGGCTATTTAC9170.10385192097740854No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCCGCGTCAGTAATACCTTC9110.10317241004407761No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGTTACCAGATGTGACAT9050.1024928991107467No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGGGACAGAGTCACCATCACCTGCCG8980.10170013635519397No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8830.10000135902186666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00786007950.8939323
CGCGTAG1450.044.54990428
GACGCGT1450.044.5120126
CGTATGC1400.044.4029941
TCGTATG1500.043.6646140
GCGTAGT1500.043.10404629
ACGCGTA1500.043.04780227
AGACGCG1500.043.01363825
CGTAGTA1650.039.22560530
TTAACGG350.001398388639.13317535
GAGACGC1650.039.0966624
ATGCCGT1650.037.82791544
ACCCAGG11600.036.3991870
CTTATAC1800.035.8243941
ATCTCGT1850.035.23544737
TAGTAAT1850.035.04278632
CCCACGA1850.034.85024319
ATCTCCG1850.034.8423510
GCCGTCT1800.034.80452746
CTTGAAA1850.034.7067357
Produced by FastQC (version 0.11.5)