FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882988
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAATAGCCGTGTCATCAGTTCTCAGGTTGCTCATTTGAAGATATA35570.40283673164301215No Hit
ATTTACTACTGTGCGACCGACTATGGTTCGGGGAGCAGAAAACAACGTGT29050.3289965435543858No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22390.25357082995465396No Hit
CTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATTCCGGCAACACAGTATATCTTCAAATGAG19470.22050129786588263No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19440.22016154239921723No Hit
ATACTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATT19350.21914227599922081No Hit
GTATAGTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCC18890.21393269217701716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17360.19660516337707876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17030.19286785324375869No Hit
CTCCTGTGCAGCCTCTGGATTCCGCGTCAGTAATACCTTCATCAACTGGGTCCGCCAGGCTCCAGGGAAGGGC16730.1894702985771041No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16730.1894702985771041No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15870.1797306418660276No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15870.1797306418660276No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15790.17882462728825307No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAAC15710.1779186127104785No Hit
GGTACATACTATACAGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAATTCCGGCAACACAGTATATCTT15330.17361504346604936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14470.16387538675497287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13980.1583260474661037No Hit
GTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCCCTTCC13970.1582127956438819No Hit
GGCCTGGAGTGGGTCTCAATTATTTATAGCGGTGGAGGTACATACTATAC13790.15617426284388916No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13770.15594775919944553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13440.15221044906612546No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGTTGATGAAGGTATTAC13420.15198394542168184No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13210.14960565715502364No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13150.1489261462216927No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12770.14462257697726355No Hit
GTGGGGGACAGAGTCACCATCACCTGCCGGGCGAGTCGGGGCATCGATAATTATTTGGCCTGGTATCAGCAAAGA12410.14054551137727805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC12390.14031900773283443No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12260.1388467340439508No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12220.13839372675506348No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11960.1354491793772962No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11740.13295763928841614No Hit
GTCTGTATAGTATGTACCTCCACCGCTATAAATAATTGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGAC11530.13057935102175794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11490.13012634373287066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11330.12831431457732156No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTCGCTATTTTAAAAGGTGGCCA11270.12763480364399063No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11130.12604927813288516No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG11130.12604927813288516No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11050.1251432635551106No Hit
GTCTGCATCTGTGGGGGACAGAGTCACCATCACCTGCCGGGCGAGTCGGG10770.12197221253289964No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10660.12072644248845965No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10480.1186879096884669No Hit
GTGTGAGGCACAATTGGTGGAGTCGGGGGGAGGCCTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC10470.11857465786624508No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10460.11846140604402325No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCTGGGTCCCCCGGCCCCAGTAGTCGACACGTTGTTTT10440.11823490239957961No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10430.11812165057735778No Hit
GTTCTCAGGTTGCTCATTTGAAGATATACTGTGTTGCCGGAATTGTCTCT10380.1175553914662487No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10210.11563011048847775No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10200.11551685866625595No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT10170.11517710319959049No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG10060.11393133315515047No Hit
CTATGACAGAGCCCCGCTCACCTTCGGCGGGGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCAT10020.11347832586626318No Hit
GTATATCTTCAAATGAGCAACCTGAGAACTGATGACACGGCTATTTACTA10010.11336507404404138No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9970.11291206675515408No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9900.11211930399960136No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCGACTTATTACTGTCACAACTATGACAGAGCC9890.11200605217737954No Hit
GTGCATCCACTTTGCAATCAGGGGTCCCGTCTCGATTCAGCGCCAGTGGG9770.1106470303107177No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9760.11053377848849588No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9740.11030727484405223No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9690.10974101573294315No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9600.10872174933294677No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9600.10872174933294677No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG9520.10781573475517221No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9510.1077024829329504No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9460.10713622382184128No Hit
GTGGATGCACCATAGATCAAGAGTTTAGGAACTTTCCCTGGTCTTTGCTGATACCAGGCCAAATAATTATCGATG9450.10702297199961948No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9420.10668321653295401No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9390.10634346106628856No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC9360.10600370559962309No Hit
CCTGAGAACTGATGACACGGCTATTTACTACTGTGCGACCGACTATGGTTCGGGGAGCAGAAAACAACGTGTCGA9330.10566395013295764No Hit
CTGTCATAGTTGTGACAGTAATAAGTCGCAATATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC9120.10328566186629942No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9120.10328566186629942No Hit
GCACTGGGCTTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAACACAGA8870.10045436631075394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA150.002503924567.9437727
TTCGACA350.00487047657.43500570
TACGTAT502.9649585E-453.60600370
GTGGTAT2200.040.0643921
TCTGGAG11250.039.31107370
GTACGTA450.001479550838.68532669
GTATCAA16300.035.9804341
ACCCAGG10700.031.93815870
TATCAAC18900.030.4770932
TATACAC450.005056730530.1083033
ATCAACG19250.030.0887553
CAACGCA19450.029.9535055
TCAACGC19550.029.6270334
TGCACAG6000.029.03658770
ATTGAGT500.00666061728.45766456
AACGCAG20650.028.3768966
CGCAGAG21000.027.9023678
ACTCGTA1553.274181E-1127.72601159
TCTATTC500.00845930227.0974733
ACGCAGA21650.027.0661857
Produced by FastQC (version 0.11.5)