FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695680
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54620.32211266276655975No Hit
GGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTCACAGTGGTGGCACAAACTATG53690.31662813738441214No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT47580.28059539535761463No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43210.25482402340064164No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG37390.22050150971881488No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC37320.22008869598037364No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36770.21684515946404984No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36710.21649131911681446No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGTTGCTGCTTGTATATGAGCTG36250.21377854312134367No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33620.19826854123419516No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30250.1783945083978109No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT29200.17220230232119269No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAAT27010.15928712964710323No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26070.15374363087374976No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25880.1526231364408379No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25700.1515616153991319No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25640.1512077750518966No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25480.1502642007926024No Hit
GTGTTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC24560.14483864880166067No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACACCTTCACCGG24080.14200792602377807No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC24030.14171305906774861No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23320.1375259482921306No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23130.13640545385921873No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA23110.13628750707680695No Hit
GTGTATTACTGTGCGAGAGATCGCCTAACCGGAACCCACTACGGTGACTT22930.13522598603510097No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22850.13475419890545387No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT22630.13345678429892432No Hit
GTCGTACCCAGTGCATATAGTAGCCGGTGAAGGTGTATGCAGAAGCCTTG22140.1305670881298358No Hit
CTATATGCACTGGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTCACAGTGGTGG21580.1272645782223061No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21480.1266748443102472No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20910.1233133610115116No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20890.12319541422909984No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20740.1223108133610115No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20570.12130826571051141No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20250.11942111719192301No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20200.11912625023589357No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20120.11865446310624646No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20030.11812370258539348No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19920.11747499528212871No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGATGTCAGTAAT19870.11718012832609927No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA19100.1126391772032459No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19060.11240328363842235No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18370.10833411964521608No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17820.10509058312889225No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17800.10497263634648046No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17530.1033803547839215No Hit
CAGCAACACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17470.10302651443668616No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG17440.1028495942630685No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16960.10001887148518589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT400.00757369851.373870
GGTATCA15300.039.0931361
GTATCAA33900.034.585871
ATCAACG39350.029.5191843
TCAACGC39250.029.5061194
TCCGAGA8600.029.4702470
TATCAAC39600.029.424672
CAACGCA39750.029.1323975
AACGCAG40550.028.4739026
TAACGGC1700.028.22116936
TATTCTA2453.614332E-927.95852770
CGCAGAG42400.027.311628
TTAACGG1800.026.62559335
ACGCAGA43700.026.5768577
AGAGTAC43350.026.55642111
CAGAGTA44450.025.89923310
TGGTATC5250.025.8829252
GCAGAGT44750.025.8773789
AAACTAT20600.024.6994568
TAGGTGG13800.024.6082025
Produced by FastQC (version 0.11.5)