FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326506
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52500.3957765739468951No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51420.3876348844257018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40620.3062179892137691No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38490.2901607682136379No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34190.2577447821570351No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32880.247869214311884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32510.24507993179073445No Hit
GAGTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATCAGGCGT31780.2395767527625205No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30700.2314350632413272No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28620.21575477231162168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28600.2156040002834514No Hit
GTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATCAGGCGTCTA27850.2099500492270672No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27150.20467302824110858No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25870.19502361843821286No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25720.19389282822693604No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25560.19268665200157406No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTTCTGCCAACAATATGATAATTAT25200.18997275549450962No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAA24670.18597729674799812No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24590.18537420863531714No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAGCTG23640.17821253729723047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23490.17708174708595364No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23080.1739909205084636No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22960.17308628833944212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22440.16916621560701572No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21520.16223070231118442No Hit
GTGCAGCCACAGTTCGTTCGATCTCCAGCTTGGTCCCCTGGCCAAAACTG20770.15657675125480022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20630.15552134705760848No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20620.15544596104352337No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20250.1526566785223738No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCAGCTTGGTCC19580.14760581557867058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19420.1463996393533086No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18860.14217802256454173No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18200.13720254563492362No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT17760.13388556101517823No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17040.12845776800104938No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAG16950.12777929387428327No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16760.12634695960666592No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTTCTGCCAACAATATGATAATTATCCCAGTTTTGG16660.1255930994658147No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16660.1255930994658147No Hit
CAATATGATAATTATCCCAGTTTTGGCCAGGGGACCAAGCTGGAGATCGAACGAACTGTGGCTGCACCAT16520.12453769526862299No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16440.123934607155942No Hit
CTCCTGATCTATCAGGCGTCTACTTTAGAAACTGGGGTCCCATCAAGGTT16400.12363306309960151No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16140.12167302673338831No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15920.12001453442351562No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15770.11888374421223877No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15740.1186575861699834No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15610.1176775679868768No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCGAACGAACTGTGGCTGCACCA15480.1166975498037702No Hit
ATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGA15450.11647139176151483No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15310.11541598756432311No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15280.11518982952206774No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15190.11451135539530163No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15020.11322979315585456No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14950.1127020910572587No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAAATGACCCAGTCTCCTTCCACC14840.11187284490232234No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14810.11164668686006697No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14750.11119437077555623No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14750.11119437077555623No Hit
ACCTTGATGGGACCCCAGTTTCTAAAGTAGACGCCTGATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTCTG14440.10885740433891744No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGCTCAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG14370.10832970224032155No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14310.10787738615581084No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATCAGGCGTCTACTTTAGAAACTGGGGTCCCAT14190.10697275398678936No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14150.10667120993044887No Hit
GGCCAAAACTGGGATAATTATCATATTGTTGGCAGAAATAAGTCGCAAAA14100.10629427986002325No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13890.10471117356423566No Hit
GCCACAGTTCGTTCGATCTCCAGCTTGGTCCCCTGGCCAAAACTGGGATA13880.10463578755015054No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13880.10463578755015054No Hit
CCCCTGAACTCCTGATCTATCAGGCGTCTACTTTAGAAACTGGGGTCCCA13590.10244959314168198No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13570.10229882111351173No Hit
ATCATATTGTTGGCAGAAATAAGTCGCAAAATCATCAGGCTGCAGGCTGC13530.10199727705717124No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13490.10169573300083075No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13430.10124341691632001No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13410.10109264488814976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)