FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326506
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50810.3830363375665093No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42090.3172997332842822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39210.2955885612277668No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38460.2899346101713826No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31480.2373151723399668No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30800.23218892338217845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28410.2141716660158341No Hit
GAGTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATCAGGCGT28320.21349319188906798No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27550.2076884688045135No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26560.20022525341008632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25900.19524977648046823No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24740.186504998846594No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24470.18446957646629566No Hit
GTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATCAGGCGTCTA24380.18379110233952958No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24140.18198183800148662No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23780.17926794149442218No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23480.17700636107186848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22530.16984468973378183No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTTCTGCCAACAATATGATAATTAT21910.1651707568605042No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21820.16449228273373812No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21050.15868755964918363No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAGCTGCTTGGCCTGGTATCAGCAGAAA20730.1562752071984597No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA20400.15378746873365065No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20370.15356131069139528No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCAGCTTGGTCC19860.14971662397305402No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAGCTG19650.14813351767726646No Hit
GTGCAGCCACAGTTCGTTCGATCTCCAGCTTGGTCCCCTGGCCAAAACTG19380.14609809529696813No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19320.1456457792124574No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18970.14300726871947808No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18950.14285649669130784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18860.14217802256454173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18660.14067030228283928No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17140.1292116281419006No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16610.1252161693953891No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16370.12340690505734614No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCGAACGAACTGTGGCTGCACCA16000.12061762253619658No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15830.11933606029674951No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15810.11918528826857927No Hit
GAGTGAATTCTGAGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15660.11805449805730242No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15490.11677293581785533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15460.11654677777559996No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15370.11586830364883385No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTATTTCTGCCAACAATATGATAATTATCCCAGTTTTGG15300.11534060155023801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15230.11481289945164212No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15120.11398365329670579No Hit
CAATATGATAATTATCCCAGTTTTGGCCAGGGGACCAAGCTGGAGATCGAACGAACTGTGGCTGCACCAT14990.11300363511359919No Hit
CTCCTGATCTATCAGGCGTCTACTTTAGAAACTGGGGTCCCATCAAGGTT14970.11285286308542894No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14510.10938510643751329No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14480.10915894839525792No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT14320.10795277216989596No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14320.10795277216989596No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14240.10734968405721496No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14150.10667120993044887No Hit
ACCTTGATGGGACCCCAGTTTCTAAAGTAGACGCCTGATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTCTG14080.106143507831853No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14030.1057665777614274No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13980.10538964769100177No Hit
GGCCAAAACTGGGATAATTATCATATTGTTGGCAGAAATAAGTCGCAAAA13940.10508810363466128No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13890.10471117356423566No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13490.10169573300083075No Hit
GTTCAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAAGCAGCTATTA13470.1015449609726605No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAG13430.10124341691632001No Hit
ATTAATAGCTGCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGA13400.10101725887406464No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13300.10026339873321342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)