FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493252
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT24600.4987308718464396No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG23240.47115875860614853No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG23030.46690129994404483No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA22600.4581836464930705No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19330.3918889330403121No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCTGGGACCC16320.3308653588834916No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13680.2773430214170444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13280.2692335763463706No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCT12740.25828582550096096No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC12180.24693260240201764No Hit
GTATATTACTGTGCGAGGCCGGCGTTCGGACGGAGTGGTGGTTCCCGCTT11810.23943136571164436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11620.23557937930307427No Hit
GTATTACCTCCGATATTGGACCTGCTTCCAGAACAAGAGATGGTGACCCT10730.21753586402082506No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10480.2124674608516539No Hit
GTGCTGACTCAGGCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10350.20983189120368492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10340.2096291550769181No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10120.20516896028804749No Hit
GAGGTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA10090.20456075190774695No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9610.19482941782293836No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9280.18813912563963248No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9180.18611176437196403No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG9120.18489534761136295No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGGCAC9090.1842871392310624No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG9000.18246251409016082No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8930.1810433612027929No Hit
GTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC8890.1802324166957255No Hit
GCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8730.17698863866745598No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8620.17475854127302068No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8430.1709065548644506No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC8400.1702983464841501No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGG8360.1694874019770827No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAA8330.16887919359678216No Hit
GTCTCTGGTGACTCCATCACCAGTAGTACTTACTACTGGGGCTGGATCCG8260.16746004070941425No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7990.16198616528670945No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7640.15489040084986985No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7590.1538767202160356No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGTTAT7510.15225483120190086No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTG7410.1502274699342324No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACACCATTCAGG7370.14941652542716502No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7120.14434812225799387No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6890.13968519134235644No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT6890.13968519134235644No Hit
CCCCTGAGGGCCGCTGATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTA6800.13786056620145484No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6790.137657830074688No Hit
GATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6730.1364414133140869No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG6540.13258942690551687No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6450.13076480176461525No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG6300.12772375986311257No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG6300.12772375986311257No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6280.12731828760957886No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6110.1238717734545425No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGCGGGAC6080.12326356507424198No Hit
ACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT6040.12245262056717458No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5940.12042525929950613No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT5920.12001978704597244No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5900.11961431479243875No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5790.11738421739800346No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5680.11515412000356817No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT5660.11474864775003447No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAAC5520.11191034197529863No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5510.11170760584853177No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC5420.10988298070763018No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC5400.10947750845409648No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC5330.10805835556672856No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5320.10785561943996173No Hit
GTCTACAGTATTACCTCCGATATTGGACCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA5220.10582825817229327No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5210.10562552204552642No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTT5150.10440910528492535No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT5140.10420636915815852No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5130.10400363303139167No Hit
CTCTTGTTCTGGAAGCAGGTCCAATATCGGAGGTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCC5100.10339542465109113No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACAC5020.10177353563695637No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT4960.1005571188763553No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC4950.10035438274958845No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC4940.1001516466228216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)