FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493252
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43230.8764282760130724No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG21540.43669361705578486No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA20740.42047472691443727No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17640.3576265276167152No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCTGGGACCC16720.3389748039541654No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG14450.29295370317809155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14390.29173728641749047No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG12960.26274602028983157No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCT12310.24956817204998663No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12020.24368882437374811No Hit
GTGCTGACTCAGGCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11630.23578211542984112No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11350.2301055038803695No Hit
GAGGTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA11240.22787540648593418No Hit
GTATATTACTGTGCGAGGCCGGCGTTCGGACGGAGTGGTGGTTCCCGCTT11160.2262535174717994No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10770.21834680852789243No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10600.21490029437285607No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10340.2096291550769181No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC10280.20841273831631704No Hit
GTATTACCTCCGATATTGGACCTGCTTCCAGAACAAGAGATGGTGACCCT10040.20354707127391275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9820.1990868764850421No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAA9600.19462668169617153No Hit
GTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9230.18712544500579825No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACACCATTCAGG9190.18631450049873088No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9140.18530081986489663No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9080.18408440310429555No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9030.18307072247046136No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8740.17719137479422284No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG8730.17698863866745598No Hit
GCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8670.1757722219068549No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGGCAC8650.17536674965332122No Hit
GTCTCTGGTGACTCCATCACCAGTAGTACTTACTACTGGGGCTGGATCCG8540.17313665225888591No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8010.1623916375402431No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7720.1565122898640046No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7630.154687664723103No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGG7530.15266030345543455No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTG7080.1435371777509265No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7050.14292896937062596No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG7050.14292896937062596No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT6970.1413070803564912No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6910.14009066359589015No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC6790.137657830074688No Hit
CCCCTGAGGGCCGCTGATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTA6790.137657830074688No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG6600.13380584366611792No Hit
GATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6580.13340037141258423No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6540.13258942690551687No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6440.13056206563784842No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6320.12812923211664626No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6260.12691281535604518No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6110.1238717734545425No Hit
ACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT6050.12265535669394143No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5950.12062799542627298No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT5950.12062799542627298No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGCGGGAC5860.11880337028537136No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGTTAT5820.11799242577830399No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5770.11697874514446976No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG5710.11576232838386868No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5600.1135322309894334No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC5520.11191034197529863No Hit
CTCTTGTTCTGGAAGCAGGTCCAATATCGGAGGTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCC5510.11170760584853177No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5460.11069392521469755No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5330.10805835556672856No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC5320.10785561943996173No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5270.10684193880612748No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTT5200.10542278591875959No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT5190.10522004979199275No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5130.10400363303139167No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACAC5110.10359816077785797No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAAC5070.1027872162707906No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA5000.10136806338342268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT5050.060.9051170
AATCGGA200.005303904456.21093463
ACCCTGG3300.045.56604470
CTACGGA5150.041.6722669
GGTATCA6000.041.1351781
ACCAACT451.2269996E-437.0286648
CTAGAGA657.1380055E-735.889324
GCGGTCA5150.034.33125323
TTCAGGG5500.033.95913341
GGATGGG4250.033.77247670
CGTGTCT5250.033.64318513
GAACTGG5300.033.50075549
TCTACGG5800.033.44927668
CGGCGGT5300.033.35284421
TGCGGCG5300.033.3460819
TGGTATC2200.033.3393142
GGTCTTA5550.033.3373355
TGATATG400.00307496733.3257983
CAGCCGT5300.033.3257989
ACAGCCG5300.033.3257988
Produced by FastQC (version 0.11.5)