FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283093
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51620.4023091077575826No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGA47930.3735504752968023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45020.3508709033561869No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37190.2898464881345312No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32890.25633371860028853No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29280.22819857952619177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28780.22430174585941937No Hit
GAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28610.22297682241271677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27930.2176771286259063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27430.2137802949591339No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26880.20949377792568424No Hit
GTCTCCATCCTCCCTGTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGCATTAGCAC25370.19772534025203162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25100.1956210500719745No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24730.19273739315856295No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA24410.1902434196118286No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24360.18985373624515137No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24030.1872818260250816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24010.1871259526784107No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23900.18626864927172077No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGCA23450.1827614989716256No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22980.17909847532485954No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22900.17847498193817596No Hit
CTGTCACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC22670.17668243845146064No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22420.17473402161807444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22280.17364290819137818No Hit
ACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21290.1659271775311688No Hit
GTCTCTGGTGGTTCCATCGGTACTTACTACTGGAGCTGGATCCGGCAGCC20660.1610171671110356No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCC20310.15828938354429492No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19550.15236619637080087No Hit
ACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG19200.14963841280406018No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19080.14870317272403483No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18630.14519602242393964No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGCAAAGCCCCTAAGCTCCTGATCTTTG18500.14418284567057882No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18490.14410490899724337No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17780.13857140519042657No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17560.13685679837704673No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17420.13576568495035046No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17370.1353760015836732No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17260.13451869817698328No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17200.13405107813697056No Hit
GATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAATTTAAAT17080.1331158380569452No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16950.1321026613035844No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16780.13077773785688176No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA16480.12843963765681832No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16460.12828376431014743No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16180.1261015374567549No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15970.12446486731671048No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15550.12119152703662167No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15340.11955485689657726No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCGGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA15120.1178402500831974No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14900.11612564326981756No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14610.11386547974308954No Hit
CTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14580.11363166972308322No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14550.11339785970307686No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14450.11261849296972239No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14340.11176118956303246No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCCA14330.111683252889697No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14240.11098182282967797No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTG14150.11028039276965894No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14070.10965689938297535No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13960.10879959597628543No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTT13900.10833197593627274No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13870.10809816591626639No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG13830.10778641922292459No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13820.10770848254958916No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAG13740.10708498916290557No Hit
GCCCCTAAGCTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT13700.10677324246956378No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13620.10614974908288019No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13600.1059938757362093No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13510.10529244567619025No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGA13510.10529244567619025No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13470.10498069898284847No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13260.10334402884280407No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13160.10256466210944958No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13160.10256466210944958No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13000.10131767533608242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG150.002196722770.2257153
GCGTATG150.00235138169.0312240
CGCGTAT200.00737935651.71422639
TAGTGCC200.00771855451.1297267
AGTAGCC200.00772093351.125742
GTGGTAT3150.043.281051
CGAGACG2850.041.87854823
GACGTAG2900.041.19183726
TCGTATG2100.041.09000840
CGTATGC2200.040.8459841
AGACGTA3000.039.8063525
AATCGCG350.00137727539.25585636
CTTATAC3250.038.8031271
ACGTAGA3100.038.54633327
ACGAGAC3200.038.35925322
GTAGTTT1056.6349203E-638.23181570
TGTCGTA557.4947166E-637.56296538
ATGCCGT2450.036.82646644
GCCGTCT2100.036.43673746
GTATCAA30100.036.3485371
Produced by FastQC (version 0.11.5)