FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283093
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49270.38399398952375235No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGA44460.34650644964940186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33750.263036272507137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32170.25072227812013625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30210.23544669014638844No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29600.23069255307292613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28800.22445761920609025No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28060.21869030537926712No Hit
GAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27020.2105848913523805No Hit
GTCTCCATCCTCCCTGTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGCATTAGCAC22940.17878672863151776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22940.17878672863151776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22570.17590307171810618No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21920.17083718795130207No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGCA21910.1707592512779666No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21570.16810940438456137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21540.16787559436455501No Hit
CTGTCACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC21530.16779765769121957No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21420.16694035428452966No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA21000.16366701400444084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20420.15914668695098483No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20370.1587570035843076No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20360.15867906691097217No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20180.1572762067909341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20090.15657477673091505No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20090.15657477673091505No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCC19730.15376905649083894No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19630.15298968975748445No Hit
GTCTCTGGTGGTTCCATCGGTACTTACTACTGGAGCTGGATCCGGCAGCC19420.15135301961744005No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19150.14924872943738296No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18760.1462091991773005No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18730.14597538915729413No Hit
ACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18650.14535189577061053No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18450.1437931623039016No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17810.13880521521043293No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGCAAAGCCCCTAAGCTCCTGATCTTTG17630.13740235509039486No Hit
ACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG17240.1343628248303124No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17150.13366139477029335No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA16360.12750439757679297No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16360.12750439757679297No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15890.12384137393002691No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15870.123685500583356No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15730.12259438715665973No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15720.12251645048332428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14990.11682707332983658No Hit
GATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAATTTAAAT14840.11565802322980485No Hit
CTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14820.11550214988313395No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14560.11347579637641231No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14470.11277436631639327No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCCA14470.11277436631639327No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14360.11191706290970334No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14240.11098182282967797No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14060.10957896270963992No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14060.10957896270963992No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14060.10957896270963992No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14050.10950102603630447No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCGGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA14040.109423089362969No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13920.10848784928294364No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13680.10661736912289288No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13580.1058380023895384No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13350.1040454589028231No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13320.10381164888281676No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13200.10287640880279139No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13040.10162942202942421No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13010.10139561200941787No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGA13000.10131767533608242No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12910.10061624527606337No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAG12880.10038243525605706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG451.08873544E-437.7942433
GTATCAA28800.037.1275831
GTGGTAT3250.035.6250151
TCTATAC400.002783155534.0148163
TAGTGGC400.002790078833.99758511
TATCAAC32050.032.8109472
ATCAACG32550.032.3950653
TCAACGC32950.032.093774
CAACGCA34050.031.1568265
AACGCAG34400.030.740986
ACGCAGA36200.029.2124237
TGTTAGA15350.028.82152470
CGCAGAG37050.028.6340088
GATTCAC3000.028.377771
GGTACGG605.9030985E-428.3357374
CGAGAGC500.007128936228.06540352
AGAGTAC37800.027.79167711
GCAGAGT38300.027.5208459
ATTCACT3050.024.5477222
AATAGTC1401.4577381E-824.52966936
Produced by FastQC (version 0.11.5)