FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897884
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41020.45685188732620247No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38730.43134747918439353No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG37050.4126368216829791No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35470.3950398937947441No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33120.36886724788502745No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29190.32509767408707585No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28520.31763568567877365No Hit
GATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA26000.289569699426652No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23720.264176664246161No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23690.26384254536220714No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23340.25994449171607914No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC22870.2547099625341358No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22500.2505891629653719No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAATACCCAGGCAAAGC21710.24179069902125438No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21690.2415679530986185No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC21520.2396746127562135No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20630.22976241919891657No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20200.224973381862245No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAATA18510.20615135139951266No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTGT17980.20024858444966162No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGACGGTCAGGGAGG17690.19701876857144132No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17650.1965732767261695No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17610.19612778488089774No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17580.19579366599694392No Hit
TCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATAT17250.1921183582734518No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17020.18955678016313912No Hit
GCTTATAACTATGTCTCCTGGTATCAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG16790.18699520205282644No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTG16650.18543598059437522No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16490.18365401321328811No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16280.18131518102561134No Hit
GTCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15880.1768602625728936No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15120.16839591751272992No Hit
CCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT15060.16772767974482228No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14570.16227040464024306No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14430.16071118318179187No Hit
CAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG14410.16048843725915599No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAA14150.15759274026488945No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13960.15547665399984853No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTATTGTTGATACCAGGAGA13820.15391743254139734No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13810.1538060595800794No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13680.15235821108294612No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAACTGGGACCAAGGT13650.1520240921989923No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTGTTGCCTGCATATGAG13640.15191271923767435No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13240.14745780078495663No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG12880.1434483741775107No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12790.14244601752564917No Hit
ACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT12600.14032993126060828No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12600.14032993126060828No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG12480.13899345572479296No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA12410.13821384499556735No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12330.13732286130502383No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12310.13710011538238792No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12030.1339816724654855No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11820.1316428402778087No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGA11810.13153146731649076No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11750.13086322954858312No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11700.1303063647419934No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11530.12841302439958838No Hit
GACATAAGGGATGGTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCT11380.12674242997981922No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11350.1264083110958654No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11340.12629693813454743No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11320.12607419221191155No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11230.12507183556005008No Hit
CCCTGACCGTCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCC11120.12384673298555271No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11060.12317849521764504No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11000.12251025744973737No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10960.12206476560446561No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10890.12128515487524001No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10830.12061691710733234No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10810.12039417118469646No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10790.12017142526206058No Hit
GTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGTCTG10760.11983730637810674No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10300.11471415015748138No Hit
CTCCCTGACCGTCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCAT10290.11460277719616341No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10200.11360042054430193No Hit
GCCTCATCCTCAGTCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC10020.11159570724057896No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9830.10947962097553805No Hit
ACACCATCCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCA9820.10936824801422011No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9780.10892275616894832No Hit
GTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG9770.10881138320763038No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9500.10580431325204592No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG9400.10469058363886648No Hit
GGGTATTGTTGATACCAGGAGACATAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGA9250.10301998921909736No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG9220.10268587033514351No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC9160.10201763256723585No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG9120.10157214072196409No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9050.10079252999273848No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA9050.10079252999273848No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC9010.10034703814746672No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8990.10012429222483081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG200.006922801552.55489349
CGTATGC1250.052.47658541
TCGTATG1300.050.401140
ATGCCGT1350.048.8720744
TGTCGCC352.5153277E-548.44659410
CCCACGA1500.047.4776619
CACGAGA1550.045.9512421
ACGAGAC1500.045.22185522
TCTACAT1600.044.73704532
ATCTCGT1500.043.46188437
CATCTCG1500.043.39097636
TACATCT1600.042.71670534
GCCGTCT1600.041.37502346
CCGTCTT1700.041.05887247
CGAGCCC1750.040.6928715
AACAATT700.001403634939.09916370
CGAGACC1750.038.76375223
CTGTCGC350.001466342238.757289
GCCCACG1850.038.49754718
CTTATAC1900.037.4928051
Produced by FastQC (version 0.11.5)