FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897884
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41610.46342289204396114No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG38080.4241082366987272No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36510.40662268177181016No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35870.3994948122474618No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30570.34046714274895196No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29950.33356201914723954No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28390.3161878371816404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26520.295361093415185No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC26000.289569699426652No Hit
GATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA25230.28099398140517035No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22610.2518142655398693No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22280.24813895781637718No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22010.2451318878607927No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21850.2433499204797056No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAATACCCAGGCAAAGC21770.24245893678916208No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21550.2400087316401673No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT21270.23689028872326492No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC20500.2283145707017833No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18820.2096039132003689No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAATA18100.20158505998547696No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17630.19635053080353362No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17410.1939003256545389No Hit
GCTTATAACTATGTCTCCTGGTATCAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG16630.18521323467173934No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTGT16420.18287440248406253No Hit
TCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATAT16340.18198341879351898No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16260.18109243510297543No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16170.18009007845111394No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15940.17752850034080125No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15020.1672821878995505No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGAGACGGTCAGGGAGG14860.16550022051846341No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14750.16427511794396604No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTG14730.16405237202133013No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14710.16382962609869425No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14640.16305001536946864No Hit
CCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT14510.16160216687233542No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14450.16093392910442775No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT14450.16093392910442775No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14130.15736999434225357No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGATGGTGTTGCCTGCATATGAG13880.15458567030930498No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAACTGGGACCAAGGT13680.15235821108294612No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCCCTTATGTCTTCGGAA13360.14879427632077197No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG13290.14801466559154636No Hit
CAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG13250.14756917374627457No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13100.14589857932650543No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTATTGTTGATACCAGGAGA13100.14589857932650543No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12850.14311425529355684No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12700.1414436608737877No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12670.1411095419898339No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12410.13821384499556735No Hit
ACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT12350.1375456072276597No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12130.13509540207866494No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11800.13142009435517282No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11740.13075185658726515No Hit
GTCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11510.1281902784769525No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC11140.12406947890818859No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11070.12328986817896298No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC10940.12184201968182973No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10940.12184201968182973No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGA10890.12128515487524001No Hit
CCCTGACCGTCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCATCC10700.1191690686101991No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10670.11883494972624525No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10590.11794396603570172No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10570.11772122011306584No Hit
GACATAAGGGATGGTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCT10540.11738710122911201No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG10530.11727572826779405No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10520.1171643553064761No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10510.11705298234515817No Hit
GTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGTCTG10430.11616199865461463No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10340.11515964200275314No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10280.11449140423484547No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10160.11315492869903017No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10140.11293218277639429No Hit
CTCCCTGACCGTCTCTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACACCAT10120.11270943685375838No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10070.11215257204716868No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10020.11159570724057896No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9970.11103884243398926No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9960.11092746947267129No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9850.10970236689817393No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9690.10792039951708685No Hit
GGGTATTGTTGATACCAGGAGACATAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGA9450.10524744844545621No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9370.10435646475491266No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9250.10301998921909736No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG9170.1021290055285538No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA9140.10179488664459997No Hit
GTATTGTTGATACCAGGAGACATAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA9060.10090390295405642No Hit
CTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9040.10068115703142054No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG8990.10012429222483081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAG200.00772474951.11806532
AATGGTA3750.037.7116670
GTGGTAT3000.035.0164681
CTCGCTA601.51523145E-533.89446323
TCAACGC26450.033.544184
ATCAACG26350.033.4144483
AACGCAG27100.032.9913756
CAACGCA26900.032.984875
TAGGCAA2707.2759576E-1231.42638270
ACGCAGA28250.031.40867
CGCAGAG28300.031.3548558
AGAGTAC28150.031.28130711
CAGAGTA28250.031.1705810
GCAGAGT28700.030.7998499
AATCGTT703.9144026E-529.49963239
TAATCGT703.9599785E-529.44962938
GTATTAT704.35101E-529.0459181
AGCGTAC500.00606650429.00723863
GTGGCCG4150.028.96528270
GAATACG1203.268724E-928.223369
Produced by FastQC (version 0.11.5)