FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314369
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46460.35347760027815633No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA41060.3123932472540056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36270.27594990447887924No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34060.25913575259306937No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30810.234409058643349No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA28980.2204860278962757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28860.2195730422735168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27910.21234523942667546No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27420.20861721480040993No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27060.2058782579321332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25310.1925638842668992No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24720.18807503828833455No Hit
GAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24340.18518391714959803No Hit
GCATAGATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAATT22990.17491282889356033No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22760.17316293978327243No Hit
GTCTCCATCCTCCCTGTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAC21990.16730461537056945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21920.1667720404239601No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21630.16456565850229274No Hit
CTGTCACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC21380.162663605121545No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC21120.16068546960556737No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21070.16030505892941785No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20500.15596837722131304No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20430.15543580227470366No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGCA20420.15535972013947377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20330.1546749809224046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20070.15269684540642695No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACACCTATTTAAATTGGTATCAGCAGAAA19390.14752326021079315No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19190.146001617506195No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT19180.1459255353709651No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18920.14394739985498745No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCC18900.14379523558452764No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18790.14295833209699862No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18750.142654003556079No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18730.1425018392856192No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18580.14136060725717056No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17450.13276332597619087No Hit
ACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA17330.13185034035343193No Hit
GATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAATTTAAAT17260.13131776540682258No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG17160.1305569440545235No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTT17140.1304047797840637No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG16950.12895921921469541No Hit
GTCTCTGGTGGTTCCATCGGTACTTACTACTGGAGCTGGATCCGGCAGCC16530.12576376953503926No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16520.12568768739980934No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT16120.12264440199061298No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15880.12081843074509518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15560.1183838024177381No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15350.11678607757791No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15300.11640566690176046No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15280.11625350263130063No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15240.11594917409038102No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15210.11572092768469128No Hit
CATTAACACCTATTTAAATTGGTATCAGCAGAAACCAGGCAAAGCCCCTA15170.11541659914377164No Hit
ACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG15100.11488402419716227No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCA15010.11419928498009312No Hit
AGTCAGAACATTAACACCTATTTAAATTGGTATCAGCAGAAACCAGGCAAAGCCCCTAAGCTCCTGATCTATG14980.11397103857440338No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14770.1123733137345753No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGGCACTGTCACTCTGTTGACAG14540.1106234246242874No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14450.10993868540721821No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14390.10948219259583876No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14250.10841704270262004No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14230.10826487843216023No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14110.10735189280940131No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14060.10697148213325176No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA13900.10575416796957324No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTGACAGTGCCCCTCGGACGTTCGGCCAAGGGA13600.10347170391267596No Hit
CTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAA13330.10141748626146845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)