FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161226
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCT85940.7400798810911916No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73360.6317461028258065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66660.574048462573177No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAA59400.5115283329859993No Hit
GTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAAAAGACT58410.5030028607695659No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50230.4325600701327734No Hit
CTTTTATACGTCTCCAACAATAGGGACTACTTAGCTTGGTACCAGCACAA45440.391310563146192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45370.3907077519793735No Hit
GTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA45220.3894160137647624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41900.36082554128136984No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41720.35927545542383654No Hit
GTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA39320.33860764399005877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38630.3326656482028477No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA38380.3305127511784958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37340.3215566995571921No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36330.3128589955788107No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36260.31225618441199215No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36030.31027551914958845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34390.2961525146698403No Hit
GTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT32490.27979049728476624No Hit
GCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT32370.2787571067130774No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32290.2780681796652848No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31030.26721757866255147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30790.2651507975191737No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACG29160.25111390892039964No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29130.2508555612774774No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATA28710.24723869427656633No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27640.23802429501234038No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAA27070.2331156897968182No Hit
GGCCAAAAGTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCC26880.23147948805831078No Hit
CAATAGGGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGC26800.2307905610105182No Hit
ACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATT26610.22915435927201078No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACT26570.22880989574811447No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT26320.22665699872376263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26300.22648476696181452No Hit
GTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26020.22407352229454042No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26010.22398740641356635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC25950.2234707111277219No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25290.21778706298343303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25170.21675367241174412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25010.21537581831615896No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG24260.20891712724310343No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24080.20736704138557008No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23110.19901380093108492No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21790.18764650464250715No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGA21530.1854074917371812No Hit
GCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATT21350.18385740587964788No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21300.1834268264747775No Hit
CTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATC21270.18316847883185527No Hit
GTCAGAGACCCCGGATTCCCGGGCAGATGCCCAGGTAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTA20960.180498886521659No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20830.17937938006899604No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20500.17653755599685161No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20390.1755902813061368No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19930.1716289507813294No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAG19750.17007886492379606No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAACAATAGGGACTA19360.16672034556580717No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18830.16215620387418125No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18290.15750594630158124No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAAC18270.15733371453963313No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18250.15716148277768496No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18120.15604197632502198No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18040.1553530492772294No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17690.1523389934431368No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17650.1519945299192405No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17640.15190841403826644No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17550.1511333711094998No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17450.15027221229975907No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17150.14768873587053682No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16970.1461386500130035No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16840.14501914356034054No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16820.14484691179839237No Hit
TTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16760.14433021651254796No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16580.14278013065501463No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16540.14243566713111833No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16520.14226343536917016No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16510.1421773194881961No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16290.14028277010676649No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16150.13907714777312943No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16140.13899103189215534No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16130.13890491601118127No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16090.138560452487285No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15980.13761317779657017No Hit
GTATAAAAGACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA15830.13632143958195905No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15520.13365184727176277No Hit
GGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGC15480.13330738374786646No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG15400.13261845670007388No Hit
GGTAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTA15350.1321878772952035No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGAT15310.1318434137713072No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAA15190.13081002319961832No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15070.12977663262792946No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14780.12727927207968132No Hit
CTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAACAATAGGGACTACT14700.1265903450318887No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14570.12547083857922575No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14430.12426521624558871No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14200.12228455098318501No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13950.12013165395883317No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13830.11909826338714427No Hit
GGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGAT13730.11823710457740354No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13640.11746206164863689No Hit
GCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGT13610.11720371400571465No Hit
GCTAAGTAGTCCCTATTGTTGGAGACGTATAAAAGACTCTGACTGGACTT13530.11651478695792206No Hit
TTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACT13260.11418965817162206No Hit
GTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTC13090.1127256881950628No Hit
ACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAACAATAG12960.11160618174239985No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12930.11134783409947763No Hit
GCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC12830.11048667528973687No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA12690.10928105295609984No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12640.10885047355122948No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG12630.10876435767025541No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12590.10841989414635911No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12580.10833377826538504No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12470.10738650357467022No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12400.1067836924078517No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC12240.10540583831226652No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACC12130.10445856362155172No Hit
GTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAAAAGAC12020.10351128893083689No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11860.1021334348352517No Hit
GAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11800.10161673954940727No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG11680.10058334897771837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.049.4563261
TACGTAT400.009473721548.5432770
GTGGTAT2600.042.9966551
TGGTATC3100.037.148092
CCGTCTT1900.036.75148847
GCCGTCT1950.035.73932646
GTATCAA27050.035.0658871
CGTATGC1800.034.5427841
TCGTATG1700.034.4776640
GCGATCT1400.034.1913534
ATGCCGT1850.033.7992344
GGTCTGG36200.033.43496370
CGTCTTC2100.031.6562448
GAGCGAT2050.031.61668632
CCAACCG450.004315369331.10086848
GTAATAC553.628469E-430.7911973
CGGGAAC553.628469E-430.7911974
CGAGACA2350.030.295823
TATCAAC31400.030.202832
ACGAGAC2250.030.13030622
Produced by FastQC (version 0.11.5)