FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161226
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74570.6421661244236695No Hit
CTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCT70850.610131016701314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56320.4850046416459845No Hit
GTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAAAAGACT52170.44926655104174384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51070.4397938041345957No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAA45980.395960820718792No Hit
GTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA44270.3812350050722254No Hit
GTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT43910.37813483335715875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43430.37400127107040315No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG41970.3614283524481884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41540.3577253695663032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40830.35161114201714394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39820.3429134380387625No Hit
GTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA38710.3333545752506403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36570.31492577672218847No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35870.3088976650540033No Hit
CTTTTATACGTCTCCAACAATAGGGACTACTTAGCTTGGTACCAGCACAA34600.29796094817029584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34460.29675532583665887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32770.28220174195204034No Hit
GGCCAAAAGTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCC32380.2788432225940515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31920.27488189206924407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31600.2721261838780737No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30780.26506468163819963No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30630.26377294342358854No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30230.26032830818462555No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30100.2592088017319626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29860.2571420205885848No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28730.24741092603851447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27490.23673255679772928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27120.23354626920168856No Hit
GCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT26940.23199618334415525No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACG26400.2273459257715552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25970.22364294288967004No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATA25620.22062888705557748No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACT25290.21778706298343303No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACC25170.21675367241174412No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24940.21477300714934044No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24610.211931183077196No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACCCCG23910.20590307140901082No Hit
GTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC23220.19996107562179974No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAA23030.1983248738832923No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22320.19221064633413307No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT22290.19195229869121083No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22220.19134948752439232No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAG22120.19048832871465157No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21470.18489079645133677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21420.18446021704646642No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20960.180498886521659No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20870.17972384359289234No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20570.17714036716367013No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20390.1755902813061368No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACCCCGGATTCCCGGGC20210.17404019544860347No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19620.16895935847113308No Hit
CAATAGGGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGC19360.16672034556580717No Hit
GTCAGAGACCCCGGATTCCCGGGCAGATGCCCAGGTAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTA19000.1636201738507405No Hit
ACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATT18800.16189785623125902No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18660.160692233897622No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18620.1603477703737257No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18410.15853933687327015No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17910.15423354282456644No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17750.15285568872898128No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGA17690.1523389934431368No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17620.1517361822763183No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17270.14872212644222574No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17230.14837766291832943No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17170.14786096763248496No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17120.14743038822761462No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16940.1458803023700813No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16540.14243566713111833No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16460.14174674008332572No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16370.14097169715455907No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16200.1395077271779998No Hit
GTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTC16170.13924937953507757No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAAC16050.1382159889633887No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG15920.13709648251072573No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15900.1369242507487776No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15890.13683813486780352No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACGTCTCCAACAATAGGGACTA15880.13675201898682943No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15730.13546028077221833No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15710.13528804901027017No Hit
GCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATT15590.1342546584385813No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGAT15350.1321878772952035No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15310.1318434137713072No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15210.13098225496156649No Hit
GAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15120.13020721203279984No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14820.1276237356035776No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14770.12719315619870725No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14730.12684869267481094No Hit
TTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14670.12633199738896647No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14540.12521249093630352No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14510.1249541432933813No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14470.12460967976948502No Hit
CTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTACCTGGGCATCTGCCCGGGAATC14470.12460967976948502No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14240.12262901450708132No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13780.11866768398227391No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAA13640.11746206164863689No Hit
GGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGAT13590.1170314822437665No Hit
GCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC13550.11668701871987021No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13520.11642867107694797No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13240.11401742640967391No Hit
AAAGTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATC13210.11375907876675169No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13130.11307015171895911No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC12780.11005609588486652No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12730.10962551647999615No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12730.10962551647999615No Hit
GGTAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTA12720.10953940059902206No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA12390.10669757652687763No Hit
GGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGC12350.10635311300298132No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12220.10523360655031837No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12200.10506137478837022No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGT12190.10497525890739615No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12190.10497525890739615No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC12170.104803027145448No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12130.10445856362155172No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12060.10385575245473319No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG12050.10376963657375911No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG12000.10333905716888873No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG11990.10325294128791468No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG11830.10187508719232947No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11760.10127227602551098No Hit
GCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCC11760.10127227602551098No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG11710.10084169662064059No Hit
GTGCTGGTACCAAGCTAAGTAGTCCCTATTGTTGGAGACGTATAAAAGAC11680.10058334897771837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)