FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399803
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63530.45384957740482057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54050.38612576198222176No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46950.3354043390391362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40950.29254116472103575No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA37950.2711095775619855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35750.25539308031201535No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35680.2548930099449708No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAG35070.2505352538892973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34820.24874928829270979No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34490.24639181370521424No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32920.23517594975864461No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31470.224817349298437No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAG30080.21488738058141038No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29480.2106010631496003No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28990.20710057058028877No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28910.20652906158938078No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28870.20624330709392677No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28260.20188555103825323No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAATCTGTC27470.19624189975303669No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT27380.1955989521382652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27240.19459881140417615No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27010.19295572305531564No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26440.18888372149509608No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25990.18566898342123855No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25540.18245424534738103No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24560.17545326020875796No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCGTGGCCGAACGTC24100.1721670835110369No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCGAGGGGTA23890.1706668724099034No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22650.1618084830508293No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22200.15859374497697176No Hit
CCATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG21930.15666490213265724No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21810.15580763864629524No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21800.15573620002243174No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAAT21720.15516469103152372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21550.1539502344258442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21390.1528072164440282No Hit
GATTTCACTCTCACCATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC21230.15166419846221219No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAA20840.14887809213153566No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20770.14837802176449116No Hit
GTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAA19900.14216286148836657No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19850.14180566836904906No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAAT19790.14137703662586806No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19740.14101984350655056No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19080.1363048943315595No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT19020.1358762625883785No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19000.1357333853406515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18720.13373310387247347No Hit
AGTCAGAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATG18350.13108987478952394No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC18330.13094699754179695No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18070.12908959332134592No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17820.12730362772475842No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17710.12651780286225991No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAATTAAAT17690.12637492561453292No Hit
GAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC17640.12601773249521542No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17580.1255891007520344No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17280.12344594203612938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16930.12094559020090685No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16730.11951681772363683No Hit
CCTTGGTCCCGTGGCCGAACGTCCGAGGGGTACTGTAACTCTGTTGACAG16650.11894530873272882No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16480.11773085212704931No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16400.1171593431361413No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16330.1166592727690968No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16170.11551625478728078No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA16160.11544481616341729No Hit
GTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCACGGGA16010.11437323680546477No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15970.11408748231001077No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15940.11387316643842027No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15930.11380172781455676No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15900.11358741194296626No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15800.11287302570433125No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15750.11251583258501374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15330.10951541038274672No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15240.1088724627679752No Hit
CATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGG15160.1083009537770672No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14760.10544340882252716No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14720.10515765432707316No Hit
CAATAATACACGGCCGTGTCCGCGGCGGTCACAGAACTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTA14540.10387175909753014No Hit
ACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGA14400.10287161836344114No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14340.10244298662026013No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14220.10158572313389812No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14210.10151428451003462No Hit
CACCATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTT14190.10137140726230762No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14150.1010856527668536No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14050.10037126652821861No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14030.1002283892804916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCCC200.007611125251.3115718
CGCCATA352.3899163E-548.86990714
GTGGTAT3200.047.040651
TCGTATG1600.038.87530540
TCGCTAA1650.037.4378731
GTATCAA34050.036.5725171
CGTATGC1700.034.5820341
ACTCGCT1800.034.2762829
CACTCGC1800.034.2701528
CGCTAAT1850.033.40969532
TATCAAC37350.032.6049422
CGAGACA2100.032.59274723
ACACTCG1950.031.6260627
ATCAACG38950.031.353413
TCAACGC39500.031.0034454
CAACGCA40150.030.5867215
AACGCAG41050.029.9994546
ATGCCGT2000.029.46709344
TGGTACG754.142936E-529.25684467
GTATGCC2250.029.2194942
Produced by FastQC (version 0.11.5)