FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399803
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60470.4319893585025893No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46120.32947493325846566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44890.32068798252325503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42480.30347127417215136No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA37090.26496585590972443No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36610.26153680196427637No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35940.2567504141654218No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34880.2491779200358908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32960.23546170425409862No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAG32890.2349616338870541No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30980.22131685672912546No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAG29190.2085293430575588No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT28490.20352863938711374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28060.2004567785609832No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27660.19759923360644321No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26600.1900267394769121No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26430.18881228287123258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26190.18709775589850858No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAATCTGTC26180.18702631727464505No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26050.18609761516441956No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25490.18209705222806352No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25080.17916806864966No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24660.17616764644739297No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24520.17516750571330394No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC24380.17416736497921492No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCGTGGCCGAACGTC24000.1714526972724019No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23810.1700953634189954No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23740.16959529305195087No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23620.16873802956558887No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23370.16695206396900136No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23350.16680918672127434No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23010.16438027350991533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCGAGGGGTA21820.15587907727015873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21570.15409311167357123No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20560.14687781066335764No Hit
GATTTCACTCTCACCATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC19870.14194854561677608No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAAT19830.14166279112132207No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19810.14151991387359508No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAA19670.14051977313950606No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT19510.13937675515769005No Hit
CCATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG19350.13823373717587403No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19270.137662228184966No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA19070.13623345570769602No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAAT19050.136090578459969No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18700.13359022662474648No Hit
AGTCAGAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATG18150.12966110231225392No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT18120.12944678644066343No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCACACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18060.12901815469748243No Hit
GTTACAGTACCCCTCGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAA18010.12866096157816492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17810.12723218910089493No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17730.1266606801099869No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17500.1250175917611264No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17440.1245889600179454No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17400.12430320552249138No Hit
GAGCATTACCAGCTATTTAATTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC17370.12408888965090088No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC17310.12366025790771988No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17250.12323162616453888No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17120.12230292405431337No Hit
GTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCACGGGA16590.11851667698954782No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAATTAAAT16520.11801660662250331No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA16510.11794516799863981No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16480.11773085212704931No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16430.1173736590077318No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16310.11651639552136979No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15700.11215863946569624No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15350.10965828763047371No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15260.1090153400157022No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15260.1090153400157022No Hit
CCTTGGTCCCGTGGCCGAACGTCCGAGGGGTACTGTAACTCTGTTGACAG15170.1083723924009307No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15040.10744369029070519No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14990.10708649717138768No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14790.10565772469411766No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14460.10330025010662214No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14430.10308593423503164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14160.10115709139071712No Hit
CATCAGCAGTGTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGG14110.10079989827139961No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14110.10079989827139961No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14080.10058558239980911No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14060.1004427051520821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA100.009794226130.1464870
CGATAGA306.3618337E-445.92651437
GTGGTAT3650.044.92171
GATATAG350.001120858740.93565864
GTATCAA36650.038.3999821
TAGGCGA400.00265838634.3347333
GGAAACG951.9423128E-434.24907770
TATCAAC41100.034.232532
GACAGGT2300.032.6740231
TCAACGC43050.032.6690834
ATCAACG43050.032.5921173
CAACGCA43750.032.146385
AACGCAG44150.031.9324516
TCCGAGA7400.030.77788470
GCGATCG450.004542185530.77743342
GCGATAG450.004679892730.59010536
AATGGTA4950.030.23605270
ACGCAGA46650.030.2211727
CGCAGAG47050.029.9642458
AGAGTAC47600.029.6886711
Produced by FastQC (version 0.11.5)