FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253936
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37830.3016900384070638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32710.2608586084138265No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA31930.2546381952507943No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28520.22744382488420462No Hit
CTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27170.21667772517895648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26040.20766610098123028No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGTCCTGGT25300.20176468336502024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23790.18972260147248346No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA22120.176404537392658No Hit
GGGGAAACACTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG22020.17560704852560258No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT21680.17289558637761415No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCT21610.17233734417067537No Hit
CATCTATGCTGCATCCACCAGGACCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21600.17225759528396983No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA21510.17153985530361995No Hit
GGCCAAAAGTGTGCGGAGGCCAGTAATTATATTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG21330.17010437534292022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21250.16946638424927588No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA20790.16579793546082097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20580.16412320884000461No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAATATAATTACTGG20520.16364471551977136No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20470.16324597108624364No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20290.16181049112554388No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGC20050.1598965178446109No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19390.15463309132204514No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18720.14928991591277385No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC18400.14673795153819652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18390.146658202651491No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTT18310.14602021155784667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18050.14394674050350256No Hit
GTCTCTGGTGGCTCCATCAGCAGTCCTGATTACTACTGGGGCTGGATCCG17960.14322900052315268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17850.14235176276939174No Hit
GTTTATTACTGTCAACAATATAATTACTGGCCTCCGCACACTTTTGGCCG17750.14155427390233633No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA17720.1413150272422197No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC17590.14027829171504766No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17400.13876306286764237No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17310.1380453228872925No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA17280.13780607622717586No Hit
GAGTATTAGCAACAACTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATC16940.13509461407918744No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16900.13477561853236528No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16800.13397812966530986No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG16650.13278189636472676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16560.13206415638437688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16400.13078817419708821No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15840.12632223654157787No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15760.12568424544793355No Hit
GGATACCAGTGGTCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGG15590.12432851437393934No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14930.11906508785137358No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14890.11874609230455144No Hit
CACCAGGACCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG14720.11739036123055721No Hit
ATGTATTACTGTGTGAGAGGCTGGTATTGTGGTGCTGACTGCTACTCCGT14610.11651312347679627No Hit
GTCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTTGTTG14270.11380166132880784No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14130.11268517691493027No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14060.11212693470799148No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGGGAAACACTGATGACGCAGTC14050.11204718582128594No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13490.1075812481657756No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC13340.10638501486519249No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13320.1062255170917814No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13310.10614576820507586No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13190.10518878156460937No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12840.10239757052991541No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12820.10223807275650432No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12600.10048359724898241No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12550.10008485281545469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)