FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253936
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40570.32354123336438223No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36500.29108343647522683No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA31030.24746079544729557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27250.21731571627260082No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26510.21141429865639078No Hit
CTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24590.1961025124089268No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24460.19506577688175472No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGTCCTGGT22570.1799932372944074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22510.17951474397417413No Hit
GGGGAAACACTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG21800.17385257301808069No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCT21560.17193859973714767No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20850.1662764287810542No Hit
CATCTATGCTGCATCCACCAGGACCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20780.16571818657411544No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20730.16531944214058772No Hit
GGCCAAAAGTGTGCGGAGGCCAGTAATTATATTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG20590.16420295772671015No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20230.16133199780531063No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA20160.16077375559837184No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT19980.1593382756376721No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAATATAATTACTGG19570.1560685712827449No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA19430.1549520868688673No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA19330.15415459800181192No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGC19000.15152288474052902No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18690.14905066925265723No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18480.14737594263184087No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17800.14195301833586405No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTT17670.14091628280869198No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17420.13892256064105343No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC17330.13820482066070358No Hit
GTCTCTGGTGGCTCCATCAGCAGTCCTGATTACTACTGGGGCTGGATCCG17310.1380453228872925No Hit
GAGTATTAGCAACAACTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATC16910.1348553674190708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16450.1311869186306159No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT16240.12951219200979955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15980.12743872095545547No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15870.12656148320169452No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15710.12528550101440583No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG15610.12448801214735043No Hit
GTTTATTACTGTCAACAATATAATTACTGGCCTCCGCACACTTTTGGCCG15450.12321202996006175No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15450.12321202996006175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15390.12273353663982851No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA15200.12121830779242321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15100.12042081892536781No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14880.11866634341784589No Hit
GGATACCAGTGGTCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGG14770.11778910566408493No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14720.11739036123055721No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14470.11539663906291868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14460.11531689017621313No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14070.11220668359469702No Hit
CACCAGGACCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG13950.11124969695423051No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13830.11029271031376402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13520.10782049482589223No Hit
GTCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTTGTTG13090.10439129269755394No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGGGAAACACTGATGACGCAGTC13060.10415204603743732No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12570.10024435058886577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCT200.00737340751.72467840
CCGTCAT604.4302602E-449.41916370
GTGGTAT3700.047.4955941
GGTATCA11300.039.477281
ATACCTG350.001497397538.5935255
AACGGAT8250.037.7382770
GAGTTTA3250.037.43441
GTATCAA25100.034.7374341
GTATATC400.002873027233.794941
TGCAACG400.00287977933.77876315
AATTTCG652.1271864E-532.25620747
TATCAAC28800.030.6120052
TCAACGG8800.030.32539470
ATCAACG29750.029.6297513
TCAACGC30300.029.1998844
GAATGTA500.006092426428.98287663
TCGCTAT1052.8363502E-828.98097424
CAACGCA31100.028.5573465
CAACGGA7550.028.49564269
AACGCAG31550.028.36416
Produced by FastQC (version 0.11.5)