FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212333
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44260.3650812111853757No Hit
GTAGTAAACGGCCGTGTCAGCGACGGTCAGGCTGTTCATTTGCAGATACA37970.3131977765184978No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTCCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37460.30899101154550773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36350.2998351113101763No Hit
GTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTC32590.2688205303328376No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30990.2556228362999275No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT29270.2414353152145491No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28420.23442404025956565No Hit
GGACTGAACAGAGAGAACTAACCATGGAATTTGGGCTGAGCTGGCTTTTT27930.2303822464619869No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAC27250.2247732264980001No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCACCGTGACATC26310.21701958125366544No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTT25550.21075067658803318No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24890.20530662779945774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24460.20175974752811315No Hit
GTTTACTACTGTGCGAAGCCGTTTGATGGGAGCTACCAAGTAGGCTCCTA23900.1971405546165946No Hit
ACCTTAGACTCTATGGCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG23090.19045922201243387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22270.18369540382056745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22160.1827880623558049No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22090.18221066324186505No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21750.17940615325987166No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21270.17544684504999863No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCACCTACTTAGCCTGGTA20770.17132256566471424No Hit
GAGTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTTCCTCATCTATGATGCATC20380.1681056277441924No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20280.16728077186713552No Hit
GTTTATTACTGTCAGCACCGTGACATCCGGCTCACTTTCGGCGGAGGGAC20240.16695082951631277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20180.16645591599007864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20150.16620845922696156No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20020.16513614658678763No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19490.16076441043838616No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA19460.16051695367526908No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19030.1569700734039245No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCACCGTGACATCCGG18950.156310188702279No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18880.1557327895883392No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18850.15548533282522212No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18690.1541655634219311No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18130.14954637051041259No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17640.14550457671283384No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17110.14113284056443237No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16790.13849330175785035No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16570.13667861882832524No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGC16400.13527636383732852No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16000.13197694032910098No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15950.13156451239057257No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15450.12744023300528814No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTCCTCT15390.12694531947905402No Hit
CCCCAGCTCAGCTCCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTCTTGACACAGTCTC15240.12570803566346872No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15080.12438826626017768No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15030.12397583832164925No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15000.12372838155853218No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14890.12282104009376962No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14880.12273855450606393No Hit
GTGCTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA14800.12207866980441842No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTCTTGACACAGTC14790.12199618421671274No Hit
TACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTTCCTCATCTATGATGCATCCAACAGGGCCA14570.1201815012871876No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14540.11993404452407053No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14180.11696456336666575No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13860.11432502456008374No Hit
GATCAGGACTGAACAGAGAGAACTAACCATGGAATTTGGGCTGAGCTGGC13840.11416005338467236No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13810.1139125966215553No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13760.11350016868302686No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13750.11341768309532116No Hit
CTTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGC13610.11226288486744153No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13610.11226288486744153No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13560.1118504569289131No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13480.1111905722272676No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13220.1090459469469197No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13020.10739623519280593No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12990.10714877842968888No Hit
ACAGAAACCTGGCCAGCCTCTCAGGTTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT12950.10681883607886612No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGATCCCGGGCCCCAGTAGTCAAAGTAATAGGAGC12940.10673635049116043No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGG12780.10541658108786942No Hit
CAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12740.10508663873704668No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12690.10467421079851823No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12640.1042617828599898No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12490.10302449904440447No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTCCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12430.10252958551817033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)