FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212333
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43990.36285410031732207No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTCCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34270.2826781090673932No Hit
GTAGTAAACGGCCGTGTCAGCGACGGTCAGGCTGTTCATTTGCAGATACA32880.27121261237630256No Hit
GTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTC29510.24341496931948567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29360.24217768550390031No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28250.23302178526856893No Hit
GGACTGAACAGAGAGAACTAACCATGGAATTTGGGCTGAGCTGGCTTTTT25570.21091564776344454No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT25480.21017327747409337No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25460.21000830629868197No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24970.20596651250110323No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24730.20398685839616676No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAC24420.2014298051772904No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCACCGTGACATC23190.19128407788949076No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTT23020.18988182289849406No Hit
GTTTACTACTGTGCGAAGCCGTTTGATGGGAGCTACCAAGTAGGCTCCTA23010.18979933731078838No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22970.18946939495996562No Hit
ACCTTAGACTCTATGGCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG22420.18493268763615278No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCACCTACTTAGCCTGGTA20250.16703331510401845No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19470.1605994392629748No Hit
GAGTATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTTCCTCATCTATGATGCATC19250.15878475633344963No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18590.15334070754487422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18340.151278567852232No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCACCGTGACATCCGG18300.15094862550140928No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18220.15028874079976376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18130.14954637051041259No Hit
GTTTATTACTGTCAGCACCGTGACATCCGGCTCACTTTCGGCGGAGGGAC18040.14880400022106138No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17970.14822660110712157No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17850.1472367740546533No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17830.14707180287924193No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17720.14616446141447936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17440.14385486495872007No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17200.14187521085378357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16940.13973058557343568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16770.13832833058243899No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16320.13461647913568303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15940.13148202680286686No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTCTTGACACAGTC15910.13123457003974978No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15720.12966734387334172No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGC15650.12908994475940191No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15550.12826508888234503No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15440.12735774741758246No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15240.12570803566346872No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14840.12240861215524117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14780.12191369862900704No Hit
GTGCTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA14780.12191369862900704No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14760.12174872745359566No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14730.12150127069047861No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14710.12133629951506723No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14700.12125381392736154No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTCCTCT14360.11844930394536814No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14120.11646964984043164No Hit
CCCCAGCTCAGCTCCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTCTTGACACAGTCTC13750.11341768309532116No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13720.11317022633220411No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13230.1091284325346254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12740.10508663873704668No Hit
TACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCTCAGGTTCCTCATCTATGATGCATCCAACAGGGCCA12730.10500415314934099No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12640.1042617828599898No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12640.1042617828599898No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12490.10302449904440447No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12280.10129230170258502No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGG12130.10005501788699969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.044.757051
GTATCAA23850.035.832831
TCTGGAG16000.033.47627370
AATGGTA3600.032.8482970
TATCAAC26900.031.7529532
TCAACGC27650.031.2468874
ATCAACG27750.031.0163423
CAACGCA28400.030.540695
AACGCAG28650.030.2741896
CAGTTCG18400.029.10980470
ACGCAGA29950.028.9601217
CGCAGAG30700.028.2526258
AGAGTAC30700.028.1390711
TCGTATA2050.027.6274961
GCAGAGT32000.027.1048629
ATACACG907.4770505E-626.5605414
TACACGG1058.403749E-726.0174145
ACTCGTA2200.025.64838459
TGTTAGA10250.025.10985870
CCACTCG2250.025.01554557
Produced by FastQC (version 0.11.5)