FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800568
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33470.41807816450320273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29190.36461612255298737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24460.3055330715192213No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23210.28991915739824725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22260.27805258266630695No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA22050.27542944509398326No Hit
GATCTATAGTACGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA21170.2644372495528175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20580.25706748208771774No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19470.2432023263482927No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18180.22708876697544741No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17410.21747059587692738No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17310.21622148274724945No Hit
GTATATTACTGTGCGAGACAAAGGGGCCGTTTAGCAGCAGCTGGTTCGAA16930.21147485285447332No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16900.21110011891556993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16580.20710295690060057No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA16050.20048265731330756No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15590.19473673691678908No Hit
CCTTCCACCCTGTCTGCATCTATAGGGGACAGAGTCACCCTCAGTTGCCG15470.19323780116117556No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15380.19211359934446542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15310.19123922015369088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14500.18112140380329964No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14100.17612495128458794No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14070.17575021734568458No Hit
CTATAGATCAGGAGCTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAGGC13910.17375163633819987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13870.1732519910863287No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13820.17262743452148974No Hit
ATAGGGGACAGAGTCACCCTCAGTTGCCGGGCCAGCCAGAGTGTCAGTAATTGGTTGGCCTGGTATCAGCAGAAA13640.17037903088806947No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13560.16937974038432713No Hit
GAGTCACCCTCAGTTGCCGGGCCAGCCAGAGTGTCAGTAATTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAG13550.16925482907135933No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13100.16363381998780865No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13070.16325908604890527No Hit
GTCTGCATCTATAGGGGACAGAGTCACCCTCAGTTGCCGGGCCAGCCAGA12800.15988648059877486No Hit
GTATGATAATTGTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT12250.15301635838554625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11870.14826972849277012No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11610.1450220343556075No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11550.14427256647780076No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11450.14302345334812283No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10970.13702771032566877No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10700.13365510487553836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10570.13203125780695707No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGT10490.13103196730321473No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10430.13028249942540795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10320.12890847498276223No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10070.1257856921585674No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10070.1257856921585674No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9890.12353728852514716No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9860.12316255458624376No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9830.12278782064734038No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGGGACAGAGTCACCCTCAGTT9810.12253799802140482No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9760.12191344145656585No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9700.12116397357875908No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9690.12103906226579128No Hit
CAGTATGATAATTGTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9680.12091415095282348No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT9620.12016468307501675No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9600.11991486044908115No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9470.11829101338049984No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9430.11779136812862867No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9330.11654225499895074No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9170.11454367399146607No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9020.11267000429694916No Hit
ACTTATTACTGCCAACAGTATGATAATTGTTGCACTTTTGGCCAGGGGAC8820.1101717780375933No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8790.10979704409868993No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8750.10929739884681876No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8680.1084230196560442No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8650.10804828571714083No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8460.10567497077075276No Hit
ATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC8310.10380130107623586No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8270.1033016558243647No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8200.10242727663359015No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC8180.10217745400765456No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8180.10217745400765456No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT8060.10067851825204105No Hit
TTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC8010.10005396168720208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.041.3005371
TATTGAT7350.040.31802770
AATGGTA2850.036.9699870
ACGCTTC655.738784E-736.90836331
GTATCAA17300.035.695541
CGGTACC400.002498565534.76972645
GACAGGT1500.034.1178361
CGAGTTC552.7493763E-432.28115560
CTATTGA6650.031.4361169
TGCTCTA850.004388590330.9895470
GTGCATC450.004880652330.3269671
TATCAAC20050.030.27782
ATCAACG20250.029.9768853
TCAACGC20550.029.371484
CAACGCA20850.028.783525
AACGCAG20900.028.7146596
GAGTTTA2750.028.5349161
TCTATTG6700.028.15816168
GTAATAT6200.028.0646721
GCCGTGT6200.028.04714411
Produced by FastQC (version 0.11.5)