FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312464
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTGCCAGGCTGTCCATTTGCAGATACA47670.3632099623303953No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40660.30979897353375024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36220.27596947421034024No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34510.26294054541686473No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC31220.23787319118848213No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30820.23482548854673346No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT27260.2077009350351705No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCA26810.20427226956320327No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26060.19855782710992453No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT25590.19497677650586986No Hit
GTATATTACTGTGCGAGAGATCGCGGAGTTCGGTCCCTTGATACCTGGGGCCAGGGAAGCCTGGTCACCGTCTC25540.1945958136756513No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTA25210.19208145899620865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24750.1885766009581977No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24610.1875099050335857No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23730.18080495922173867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.17981445586317035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23590.17973826329712664No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23020.1753952870326348No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22820.17387143571176047No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGGCCACTCACTTTCGGCGGAGGGA22780.17356666544758562No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22750.17333808774945444No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21910.1669379122017823No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21100.16076631435224129No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20870.15901388533323582No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20630.15718526374818662No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20620.1571090711821429No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20150.15352802057808823No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG20080.1529946726157822No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20000.15238513208743248No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19270.1468230747662412No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18350.1398133586902193No Hit
GTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCC17800.1356227675578149No Hit
TCATAGATGAGGTGCCTGGGAGACTGGCCAGGTTTCTGTTGGTACCAGGC17320.13196552438771653No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAAC17280.13166075412354167No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGT16940.12907020687805532No Hit
CTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACACAATC16910.12884162917992417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16750.1276225481232247No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16560.1261748893683941No Hit
GTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCACCTCATCTATGATG16330.12442246034938863No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.12442246034938863No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT16140.12297480159455802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15910.12122237257555254No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15780.12023186921698424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15580.11870801789610991No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14950.11390788623535579No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14710.1120792646503066No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14700.11200307208426288No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14690.11192687951821917No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14560.11093637615965085No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCACCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT14500.11047922076338856No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14490.11040302819734485No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13690.10430762291384753No Hit
CCCAGATACCAGCGGAGAAATTGTGTTGACACAATCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCAC13660.1040790452157164No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13250.10095515000792402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG12900.056.1449670
AATCGCG709.094947E-1248.84237336
GGGCGTA306.926939E-445.139873
GTGGTAT3350.043.4555471
CGCGTAT405.1330317E-542.9369739
CTTATAC5300.042.1589361
ATCGCGT504.046371E-641.0908337
CGAGACG5700.039.8064923
ACGAGAC5700.039.80497422
CGTATGC3850.039.4074641
CTCTGGA13450.039.1685269
TATACAC5800.039.108253
ATGCCGT3650.038.00624544
GCGTATG557.5017197E-637.5579240
GCCGTCT3850.037.06142446
CGGTACC753.790592E-837.04768845
ATCTCGT3700.037.0187737
TCGTATG3750.036.723340
TTATACA6100.036.6298942
TAACGGC754.3544787E-836.4689736
Produced by FastQC (version 0.11.5)