FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312464
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50180.3823342964073681No Hit
GTAATATACAGCCGTGTCCTCGGCTGCCAGGCTGTCCATTTGCAGATACA43420.3308281217618159No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41640.31726584500603444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30160.22979677918784822No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27490.20945336405417597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26290.20031025612893003No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT26040.19840544197783713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25980.1979482865815748No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC25820.19672920552487533No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA25270.19253861439247094No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCA25060.1909385705055529No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTA24550.18705274963732338No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23780.18118592205195724No Hit
GTATATTACTGTGCGAGAGATCGCGGAGTTCGGTCCCTTGATACCTGGGGCCAGGGAAGCCTGGTCACCGTCTC22720.1731095100513233No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22370.17044277023979323No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22330.17013799997561838No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21610.1646521352204708No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21600.1645759426544271No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGGCCACTCACTTTCGGCGGAGGGA21430.16328066903168392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20710.15779480427653636No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20240.1542137536724817No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19700.15009935510612102No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19290.14697545989832864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19190.14621353423789146No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG18950.14438491265284228No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18370.13996574382230675No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18270.13920381816186958No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18210.1387466627656073No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18070.13767996684099526No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18000.13714661887868923No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17530.1335655682746346No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17520.13348937570859087No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16700.12724158529300614No Hit
CTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACACAATC16630.1267082373307001No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAAC16580.12632727450048153No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16500.12571773397213182No Hit
GTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCC16350.12457484548147606No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16120.1228224164624706No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16030.12213668336807715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15850.12076521717929024No Hit
TCATAGATGAGGTGCCTGGGAGACTGGCCAGGTTTCTGTTGGTACCAGGC15070.11482219702788038No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGT14750.11238403491448147No Hit
GTCAGAGTGTTAGCATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCACCTCATCTATGATG14590.11116495385778201No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14170.10796486608394593No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13850.10552670397054699No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13790.10506954857428472No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13750.10476477831010983No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13630.10385046751758525No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.10194565336649235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG150.002564916967.5344512
TCTGGAG13850.051.35644570
GTGGTAT3000.041.6907121
CTCTGGA14300.036.118169
TCTAGCG400.002831711433.89506528
TAAGGCG400.002883578733.7697944
TACAGCG13900.032.37524447
AATTGTC13650.032.2607163
ATTGTCT13600.032.238864
GCGTGTT13550.032.21203651
AGCGTGT13750.031.65925850
CGTCCAT553.1159702E-431.60164845
GAATTGT14350.031.32053462
TATCAAC32400.031.0752642
GGAATTG14450.031.00867761
GTGTTCT14050.030.7085553
CCGAGCG553.6933177E-430.698649
ATCAACG33050.030.5558473
TCAACGC33250.030.4689884
CAACGCA33600.030.2521065
Produced by FastQC (version 0.11.5)